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APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy.

Publication ,  Journal Article
Bruggeman, LA; Wu, Z; Luo, L; Madhavan, S; Drawz, PE; Thomas, DB; Barisoni, L; O'Toole, JF; Sedor, JR
Published in: PLoS One
2019

African polymorphisms in the gene for Apolipoprotein L1 (APOL1) confer a survival advantage against lethal trypanosomiasis but also an increased risk for several chronic kidney diseases (CKD) including HIV-associated nephropathy (HIVAN). APOL1 is expressed in renal cells, however, the pathogenic events that lead to renal cell damage and kidney disease are not fully understood. The podocyte function of APOL1-G0 versus APOL1-G2 in the setting of a known disease stressor was assessed using transgenic mouse models. Transgene expression, survival, renal pathology and function, and podocyte density were assessed in an intercross of a mouse model of HIVAN (Tg26) with two mouse models that express either APOL1-G0 or APOL1-G2 in podocytes. Mice that expressed HIV genes developed heavy proteinuria and glomerulosclerosis, and had significant losses in podocyte numbers and reductions in podocyte densities. Mice that co-expressed APOL1-G0 and HIV had preserved podocyte numbers and densities, with fewer morphologic manifestations typical of HIVAN pathology. Podocyte losses and pathology in mice co-expressing APOL1-G2 and HIV were not significantly different from mice expressing only HIV. Podocyte hypertrophy, a known compensatory event to stress, was increased in the mice co-expressing HIV and APOL1-G0, but absent in the mice co-expressing HIV and APOL1-G2. Mortality and renal function tests were not significantly different between groups. APOL1-G0 expressed in podocytes may have a protective function against podocyte loss or injury when exposed to an environmental stressor. This was absent with APOL1-G2 expression, suggesting APOL1-G2 may have lost this protective function.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

10

Start / End Page

e0224408

Location

United States

Related Subject Headings

  • Transcriptome
  • Renal Insufficiency, Chronic
  • Polymorphism, Genetic
  • Podocytes
  • Mice, Transgenic
  • Mice, Inbred BALB C
  • Mice
  • Kidney Glomerulus
  • Humans
  • Genetic Variation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bruggeman, L. A., Wu, Z., Luo, L., Madhavan, S., Drawz, P. E., Thomas, D. B., … Sedor, J. R. (2019). APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy. PLoS One, 14(10), e0224408. https://doi.org/10.1371/journal.pone.0224408
Bruggeman, Leslie A., Zhenzhen Wu, Liping Luo, Sethu Madhavan, Paul E. Drawz, David B. Thomas, Laura Barisoni, John F. O’Toole, and John R. Sedor. “APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy.PLoS One 14, no. 10 (2019): e0224408. https://doi.org/10.1371/journal.pone.0224408.
Bruggeman LA, Wu Z, Luo L, Madhavan S, Drawz PE, Thomas DB, et al. APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy. PLoS One. 2019;14(10):e0224408.
Bruggeman, Leslie A., et al. “APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy.PLoS One, vol. 14, no. 10, 2019, p. e0224408. Pubmed, doi:10.1371/journal.pone.0224408.
Bruggeman LA, Wu Z, Luo L, Madhavan S, Drawz PE, Thomas DB, Barisoni L, O’Toole JF, Sedor JR. APOL1-G0 protects podocytes in a mouse model of HIV-associated nephropathy. PLoS One. 2019;14(10):e0224408.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2019

Volume

14

Issue

10

Start / End Page

e0224408

Location

United States

Related Subject Headings

  • Transcriptome
  • Renal Insufficiency, Chronic
  • Polymorphism, Genetic
  • Podocytes
  • Mice, Transgenic
  • Mice, Inbred BALB C
  • Mice
  • Kidney Glomerulus
  • Humans
  • Genetic Variation