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In vivo detection of microRNA within plants using plasmonic nanosensors

Publication ,  Conference
Crawford, BM; Strobbia, P; Wang, HN; Zentella, R; Boyanov, MI; Pei, ZM; Sun, TP; Kemner, KM; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2019

Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically microRNA, within whole plant leaves by integrating three complementary techniques: surface-enhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The method described utilizes plasmonic nanostar-based inverse molecular sentinel (iMS) nanoprobes, which not only provide large Raman signal enhancement upon target binding, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

January 1, 2019

Volume

11082

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Crawford, B. M., Strobbia, P., Wang, H. N., Zentella, R., Boyanov, M. I., Pei, Z. M., … Vo-Dinh, T. (2019). In vivo detection of microRNA within plants using plasmonic nanosensors. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 11082). https://doi.org/10.1117/12.2529818
Crawford, B. M., P. Strobbia, H. N. Wang, R. Zentella, M. I. Boyanov, Z. M. Pei, T. P. Sun, K. M. Kemner, and T. Vo-Dinh. “In vivo detection of microRNA within plants using plasmonic nanosensors.” In Proceedings of SPIE - The International Society for Optical Engineering, Vol. 11082, 2019. https://doi.org/10.1117/12.2529818.
Crawford BM, Strobbia P, Wang HN, Zentella R, Boyanov MI, Pei ZM, et al. In vivo detection of microRNA within plants using plasmonic nanosensors. In: Proceedings of SPIE - The International Society for Optical Engineering. 2019.
Crawford, B. M., et al. “In vivo detection of microRNA within plants using plasmonic nanosensors.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 11082, 2019. Scopus, doi:10.1117/12.2529818.
Crawford BM, Strobbia P, Wang HN, Zentella R, Boyanov MI, Pei ZM, Sun TP, Kemner KM, Vo-Dinh T. In vivo detection of microRNA within plants using plasmonic nanosensors. Proceedings of SPIE - The International Society for Optical Engineering. 2019.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

January 1, 2019

Volume

11082

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering