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Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets

Publication ,  Conference
Strobbia, P; Ran, Y; Crawford, BM; Cupil-Garcia, V; Zentella, R; Wang, HN; Sun, TP; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2019

Molecular analysis has revolutionized many applications, including bio-safety, bio-engineering and biofuel research; however, there are limited practical tools for in situ detection during field work. New technology is needed to translate molecular advances from laboratory settings into the practical realm. The unique characteristics of plasmonic nanosensors have made them ideal candidates for field-ready sensing applications. Herein, we discuss the development of a fiber-based plasmonic sensor capable of direct detection (i.e., no washing steps required) of miRNA targets, which are detected by immerging the sensor in the sample solution. This sensor is composed of an optical fiber that is decorated with plasmonic nanoprobes based on silver-coated gold nanostars to detect target nucleic acids using the surface-enhanced Raman scattering sensing mechanism of nanoprobes referred to as inverse molecular sentinels. The fiber sensors were tested in extracts from leaves of plants that were induced to have different miRNA expression levels. The results indicate that the fiber sensors developed have the potential to be a powerful tool for field analysis.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

January 1, 2019

Volume

11082

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Strobbia, P., Ran, Y., Crawford, B. M., Cupil-Garcia, V., Zentella, R., Wang, H. N., … Vo-Dinh, T. (2019). Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 11082). https://doi.org/10.1117/12.2529844
Strobbia, P., Y. Ran, B. M. Crawford, V. Cupil-Garcia, R. Zentella, H. N. Wang, T. P. Sun, and T. Vo-Dinh. “Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets.” In Proceedings of SPIE - The International Society for Optical Engineering, Vol. 11082, 2019. https://doi.org/10.1117/12.2529844.
Strobbia P, Ran Y, Crawford BM, Cupil-Garcia V, Zentella R, Wang HN, et al. Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets. In: Proceedings of SPIE - The International Society for Optical Engineering. 2019.
Strobbia, P., et al. “Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 11082, 2019. Scopus, doi:10.1117/12.2529844.
Strobbia P, Ran Y, Crawford BM, Cupil-Garcia V, Zentella R, Wang HN, Sun TP, Vo-Dinh T. Inverse molecular sentinel-integrated fiber sensor for direct detection of miRNA targets. Proceedings of SPIE - The International Society for Optical Engineering. 2019.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

January 1, 2019

Volume

11082

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering