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Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes.

Publication ,  Journal Article
Wang, H-N; Crawford, BM; Norton, SJ; Vo-Dinh, T
Published in: The journal of physical chemistry. B
December 2019

MicroRNAs (miRNAs), small noncoding endogenous RNA molecules, are emerging as promising biomarkers for early detection of various diseases and cancers. Practical screening tools and strategies to detect these small molecules are urgently needed to facilitate the translation of miRNA biomarkers into clinical practice. In this study, a label-free biosensing technique based on surface-enhanced Raman scattering (SERS), referred to as plasmonic coupling interference (PCI), was applied for the multiplex detection of miRNA biomarkers. The sensing mechanism of the PCI technique relies on the formation of a nanonetwork consisting of nanoparticles with Raman labels located between adjacent nanoparticles that are interconnected by DNA duplexes. Because of the plasmonic coupling effect of adjacent nanoparticles in the nanonetwork, the Raman labels exhibit intense SERS signals. Such effect can be modulated by the addition of miRNA targets of interest that act as inhibitors to interfere with the formation of this nanonetwork, resulting in a diminished SERS signal. In this study, the PCI technique is theoretically analyzed, and the multiplex capability for detection of multiple miRNA cancer biomarkers is demonstrated, establishing the great potential of PCI nanoprobes as a useful diagnostic tool for medical applications.

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Published In

The journal of physical chemistry. B

DOI

EISSN

1520-5207

ISSN

1520-6106

Publication Date

December 2019

Volume

123

Issue

48

Start / End Page

10245 / 10251

Related Subject Headings

  • Surface Plasmon Resonance
  • Spectrum Analysis, Raman
  • Silver
  • Sensitivity and Specificity
  • Rhodamines
  • RNA, Neoplasm
  • Neoplasms
  • MicroRNAs
  • Metal Nanoparticles
  • Humans
 

Citation

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Wang, H.-N., Crawford, B. M., Norton, S. J., & Vo-Dinh, T. (2019). Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes. The Journal of Physical Chemistry. B, 123(48), 10245–10251. https://doi.org/10.1021/acs.jpcb.9b06804
Wang, Hsin-Neng, Bridget M. Crawford, Stephen J. Norton, and Tuan Vo-Dinh. “Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes.The Journal of Physical Chemistry. B 123, no. 48 (December 2019): 10245–51. https://doi.org/10.1021/acs.jpcb.9b06804.
Wang H-N, Crawford BM, Norton SJ, Vo-Dinh T. Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes. The journal of physical chemistry B. 2019 Dec;123(48):10245–51.
Wang, Hsin-Neng, et al. “Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes.The Journal of Physical Chemistry. B, vol. 123, no. 48, Dec. 2019, pp. 10245–51. Epmc, doi:10.1021/acs.jpcb.9b06804.
Wang H-N, Crawford BM, Norton SJ, Vo-Dinh T. Direct and Label-Free Detection of MicroRNA Cancer Biomarkers using SERS-Based Plasmonic Coupling Interference (PCI) Nanoprobes. The journal of physical chemistry B. 2019 Dec;123(48):10245–10251.
Journal cover image

Published In

The journal of physical chemistry. B

DOI

EISSN

1520-5207

ISSN

1520-6106

Publication Date

December 2019

Volume

123

Issue

48

Start / End Page

10245 / 10251

Related Subject Headings

  • Surface Plasmon Resonance
  • Spectrum Analysis, Raman
  • Silver
  • Sensitivity and Specificity
  • Rhodamines
  • RNA, Neoplasm
  • Neoplasms
  • MicroRNAs
  • Metal Nanoparticles
  • Humans