Skip to main content

Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions.

Publication ,  Journal Article
Lopez, V; Barinova, N; Onishi, M; Pobiega, S; Pringle, JR; Dubrana, K; Marcand, S
Published in: Genes & development
February 2015

Dicentric chromosomes are unstable products of erroneous DNA repair events that can lead to further genome rearrangements and extended gene copy number variations. During mitosis, they form anaphase bridges, resulting in chromosome breakage by an unknown mechanism. In budding yeast, dicentrics generated by telomere fusion break at the fusion, a process that restores the parental karyotype and protects cells from rare accidental telomere fusion. Here, we observed that dicentrics lacking telomere fusion preferentially break within a 25- to 30-kb-long region next to the centromeres. In all cases, dicentric breakage requires anaphase exit, ruling out stretching by the elongated mitotic spindle as the cause of breakage. Instead, breakage requires cytokinesis. In the presence of dicentrics, the cytokinetic septa pinch the nucleus, suggesting that dicentrics are severed after actomyosin ring contraction. At this time, centromeres and spindle pole bodies relocate to the bud neck, explaining how cytokinesis can sever dicentrics near centromeres.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genes & development

DOI

EISSN

1549-5477

ISSN

0890-9369

Publication Date

February 2015

Volume

29

Issue

3

Start / End Page

322 / 336

Related Subject Headings

  • Telomere
  • Saccharomyces cerevisiae
  • Mitosis
  • Developmental Biology
  • Cytokinesis
  • Chromosomes, Fungal
  • Chromosome Breakage
  • Centromere
  • Cell Nucleus Division
  • 52 Psychology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lopez, V., Barinova, N., Onishi, M., Pobiega, S., Pringle, J. R., Dubrana, K., & Marcand, S. (2015). Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions. Genes & Development, 29(3), 322–336. https://doi.org/10.1101/gad.254664.114
Lopez, Virginia, Natalja Barinova, Masayuki Onishi, Sabrina Pobiega, John R. Pringle, Karine Dubrana, and Stéphane Marcand. “Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions.Genes & Development 29, no. 3 (February 2015): 322–36. https://doi.org/10.1101/gad.254664.114.
Lopez V, Barinova N, Onishi M, Pobiega S, Pringle JR, Dubrana K, et al. Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions. Genes & development. 2015 Feb;29(3):322–36.
Lopez, Virginia, et al. “Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions.Genes & Development, vol. 29, no. 3, Feb. 2015, pp. 322–36. Epmc, doi:10.1101/gad.254664.114.
Lopez V, Barinova N, Onishi M, Pobiega S, Pringle JR, Dubrana K, Marcand S. Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions. Genes & development. 2015 Feb;29(3):322–336.

Published In

Genes & development

DOI

EISSN

1549-5477

ISSN

0890-9369

Publication Date

February 2015

Volume

29

Issue

3

Start / End Page

322 / 336

Related Subject Headings

  • Telomere
  • Saccharomyces cerevisiae
  • Mitosis
  • Developmental Biology
  • Cytokinesis
  • Chromosomes, Fungal
  • Chromosome Breakage
  • Centromere
  • Cell Nucleus Division
  • 52 Psychology