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Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.

Publication ,  Journal Article
Chin, CF; Tan, K; Onishi, M; Chew, Y; Augustine, B; Lee, WR; Yeong, FM
Published in: PLoS genetics
July 2016

Cytokinesis requires the spatio-temporal coordination of membrane deposition and primary septum (PS) formation at the division site to drive acto-myosin ring (AMR) constriction. It has been demonstrated that AMR constriction invariably occurs only after the mitotic spindle disassembly. It has also been established that Chitin Synthase II (Chs2p) neck localization precedes mitotic spindle disassembly during mitotic exit. As AMR constriction depends upon PS formation, the question arises as to how chitin deposition is regulated so as to prevent premature AMR constriction and mitotic spindle breakage. In this study, we propose that cells regulate the coordination between spindle disassembly and AMR constriction via timely endocytosis of cytokinetic enzymes, Chs2p, Chs3p, and Fks1p. Inhibition of endocytosis leads to over accumulation of cytokinetic enzymes during mitotic exit, which accelerates the constriction of the AMR, and causes spindle breakage that eventually could contribute to monopolar spindle formation in the subsequent round of cell division. Intriguingly, the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit.

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Published In

PLoS genetics

DOI

EISSN

1553-7404

ISSN

1553-7390

Publication Date

July 2016

Volume

12

Issue

7

Start / End Page

e1006195

Related Subject Headings

  • Spindle Apparatus
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Myosin Subfragments
  • Mitosis
  • Microscopy, Fluorescence
  • Membrane Proteins
  • Glucosyltransferases
  • Endocytosis
  • Echinocandins
 

Citation

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Chin, C. F., Tan, K., Onishi, M., Chew, Y., Augustine, B., Lee, W. R., & Yeong, F. M. (2016). Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit. PLoS Genetics, 12(7), e1006195. https://doi.org/10.1371/journal.pgen.1006195
Chin, Cheen Fei, Kaiquan Tan, Masayuki Onishi, YuanYuan Chew, Beryl Augustine, Wei Ren Lee, and Foong May Yeong. “Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.PLoS Genetics 12, no. 7 (July 2016): e1006195. https://doi.org/10.1371/journal.pgen.1006195.
Chin CF, Tan K, Onishi M, Chew Y, Augustine B, Lee WR, et al. Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit. PLoS genetics. 2016 Jul;12(7):e1006195.
Chin, Cheen Fei, et al. “Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.PLoS Genetics, vol. 12, no. 7, July 2016, p. e1006195. Epmc, doi:10.1371/journal.pgen.1006195.
Chin CF, Tan K, Onishi M, Chew Y, Augustine B, Lee WR, Yeong FM. Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit. PLoS genetics. 2016 Jul;12(7):e1006195.

Published In

PLoS genetics

DOI

EISSN

1553-7404

ISSN

1553-7390

Publication Date

July 2016

Volume

12

Issue

7

Start / End Page

e1006195

Related Subject Headings

  • Spindle Apparatus
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Myosin Subfragments
  • Mitosis
  • Microscopy, Fluorescence
  • Membrane Proteins
  • Glucosyltransferases
  • Endocytosis
  • Echinocandins