Skip to main content

When the checkpoints have gone: Insights into Cdc25 functional activation

Publication ,  Journal Article
Margolis, SS; Kornbluth, S
Published in: Cell Cycle
January 1, 2004

DNA-responsive checkpoints operate at the G2/M transition to prevent premature mitosis in the presence of incompletely replicated or damaged DNA. These pathways prevent mitotic entry, at least in part, by suppressing Cdc25, the phosphatase that activates Cdc2/Cyclin B. To gain insight into how checkpoint signaling controls Cdc25 function, we have carefully examined the individual steps in Cdc25 activation. We found that removal of the regulatory protein, 14-3-3, that binds to phosphorylated Cdc25 during interphase is one of the early steps in mitotic activation. Moreover, our studies unexpectedly implicated the phosphatase PP1 and the G1/S kinase Cdk2 in the process of Cdc25 activation. Here we integrate our findings and those of others to propose a model for Cdc25 activation in an effort to provide insight into novel loci of DNA-responsive checkpoint control of mitotic entry.

Duke Scholars

Published In

Cell Cycle

DOI

EISSN

1551-4005

ISSN

1538-4101

Publication Date

January 1, 2004

Volume

3

Issue

4

Start / End Page

423 / 426

Related Subject Headings

  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Margolis, S. S., & Kornbluth, S. (2004). When the checkpoints have gone: Insights into Cdc25 functional activation. Cell Cycle, 3(4), 423–426. https://doi.org/10.4161/cc.3.4.817
Margolis, S. S., and S. Kornbluth. “When the checkpoints have gone: Insights into Cdc25 functional activation.” Cell Cycle 3, no. 4 (January 1, 2004): 423–26. https://doi.org/10.4161/cc.3.4.817.
Margolis SS, Kornbluth S. When the checkpoints have gone: Insights into Cdc25 functional activation. Cell Cycle. 2004 Jan 1;3(4):423–6.
Margolis, S. S., and S. Kornbluth. “When the checkpoints have gone: Insights into Cdc25 functional activation.” Cell Cycle, vol. 3, no. 4, Jan. 2004, pp. 423–26. Scopus, doi:10.4161/cc.3.4.817.
Margolis SS, Kornbluth S. When the checkpoints have gone: Insights into Cdc25 functional activation. Cell Cycle. 2004 Jan 1;3(4):423–426.

Published In

Cell Cycle

DOI

EISSN

1551-4005

ISSN

1538-4101

Publication Date

January 1, 2004

Volume

3

Issue

4

Start / End Page

423 / 426

Related Subject Headings

  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology