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Centromere deletion in Cryptococcus deuterogattii leads to neocentromere formation and chromosome fusions.

Publication ,  Journal Article
Schotanus, K; Heitman, J
Published in: Elife
April 20, 2020

The human fungal pathogen Cryptococcus deuterogattii is RNAi-deficient and lacks active transposons in its genome. C. deuterogattii has regional centromeres that contain only transposon relics. To investigate the impact of centromere loss on the C. deuterogattii genome, either centromere 9 or 10 was deleted. Deletion of either centromere resulted in neocentromere formation and interestingly, the genes covered by these neocentromeres maintained wild-type expression levels. In contrast to cen9∆ mutants, cen10∆ mutant strains exhibited growth defects and were aneuploid for chromosome 10. At an elevated growth temperature (37°C), the cen10∆ chromosome was found to have undergone fusion with another native chromosome in some isolates and this fusion restored wild-type growth. Following chromosomal fusion, the neocentromere was inactivated, and the native centromere of the fused chromosome served as the active centromere. The neocentromere formation and chromosomal fusion events observed in this study in C. deuterogattii may be similar to events that triggered genomic changes within the Cryptococcus/Kwoniella species complex and may contribute to speciation throughout the eukaryotic domain.

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

April 20, 2020

Volume

9

Location

England

Related Subject Headings

  • Humans
  • Fungal Proteins
  • Cryptococcus
  • Centromere
  • Ascomycota
  • Aneuploidy
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 0601 Biochemistry and Cell Biology
 

Citation

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Schotanus, K., & Heitman, J. (2020). Centromere deletion in Cryptococcus deuterogattii leads to neocentromere formation and chromosome fusions. Elife, 9. https://doi.org/10.7554/eLife.56026
Schotanus, Klaas, and Joseph Heitman. “Centromere deletion in Cryptococcus deuterogattii leads to neocentromere formation and chromosome fusions.Elife 9 (April 20, 2020). https://doi.org/10.7554/eLife.56026.
Schotanus, Klaas, and Joseph Heitman. “Centromere deletion in Cryptococcus deuterogattii leads to neocentromere formation and chromosome fusions.Elife, vol. 9, Apr. 2020. Pubmed, doi:10.7554/eLife.56026.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

April 20, 2020

Volume

9

Location

England

Related Subject Headings

  • Humans
  • Fungal Proteins
  • Cryptococcus
  • Centromere
  • Ascomycota
  • Aneuploidy
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 0601 Biochemistry and Cell Biology