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The effect of intrathecal administration of glial activation inhibitors on dorsal horn BDNF overexpression and hind paw mechanical allodynia in spinal nerve ligated rats.

Publication ,  Journal Article
Zhang, X; Xu, Y; Wang, J; Zhou, Q; Pu, S; Jiang, W; Du, D
Published in: J Neural Transm (Vienna)
March 2012

Recent studies have suggested that activated glia in the spinal cord may play a vital role at different times during spinal nerve ligation (SNL)-induced neuropathic pain; therefore, glial activation inhibitors have been used as effective painkillers. Brain-derived neurotrophic factor (BDNF) is also known to be a powerful pain modulator, but it remains unclear how it contributes to the glial activation inhibitor-based treatment. This study revealed the following results: (1) intrathecal administration of minocycline (a microglial activation inhibitor) could prevent mechanical allodynia during the initiation of SNL-induced neuropathic pain, and its action was associated with the elimination of BDNF overexpression in the dorsal horn; (2) the spinal injection of fluorocitrate (an astrocytic activation inhibitor) but not minocycline could reverse mechanical allodynia during the maintenance phase of SNL-induced pain, and its action was also related to a decrease in BDNF overexpression in the dorsal horn; and (3) treatment with TrkB/Fc (a BDNF-sequestering protein) had a similar effect during both the early development and maintenance periods. These results led to the following conclusions: (1) elevated BDNF expression in the dorsal horn was required to develop and maintain neuropathic pain; (2) minocycline could only prevent mechanical allodynia in the early stages, possibly by inhibiting BDNF release from microglia; and (3) fluorocitrate could reverse existing mechanical allodynia, and its action was associated with the inhibition of BDNF upregulation induced by astrocytic activation.

Duke Scholars

Published In

J Neural Transm (Vienna)

DOI

EISSN

1435-1463

Publication Date

March 2012

Volume

119

Issue

3

Start / End Page

329 / 336

Location

Austria

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Posterior Horn Cells
  • Pain Measurement
  • Neurology & Neurosurgery
  • Neuralgia
  • Minocycline
  • Microglia
  • Male
  • Injections, Spinal
 

Citation

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Zhang, X., Xu, Y., Wang, J., Zhou, Q., Pu, S., Jiang, W., & Du, D. (2012). The effect of intrathecal administration of glial activation inhibitors on dorsal horn BDNF overexpression and hind paw mechanical allodynia in spinal nerve ligated rats. J Neural Transm (Vienna), 119(3), 329–336. https://doi.org/10.1007/s00702-011-0713-7
Zhang, Xin, Yongming Xu, Jing Wang, Quanhong Zhou, Shaofeng Pu, Wei Jiang, and Dongping Du. “The effect of intrathecal administration of glial activation inhibitors on dorsal horn BDNF overexpression and hind paw mechanical allodynia in spinal nerve ligated rats.J Neural Transm (Vienna) 119, no. 3 (March 2012): 329–36. https://doi.org/10.1007/s00702-011-0713-7.
Zhang, Xin, et al. “The effect of intrathecal administration of glial activation inhibitors on dorsal horn BDNF overexpression and hind paw mechanical allodynia in spinal nerve ligated rats.J Neural Transm (Vienna), vol. 119, no. 3, Mar. 2012, pp. 329–36. Pubmed, doi:10.1007/s00702-011-0713-7.
Journal cover image

Published In

J Neural Transm (Vienna)

DOI

EISSN

1435-1463

Publication Date

March 2012

Volume

119

Issue

3

Start / End Page

329 / 336

Location

Austria

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Posterior Horn Cells
  • Pain Measurement
  • Neurology & Neurosurgery
  • Neuralgia
  • Minocycline
  • Microglia
  • Male
  • Injections, Spinal