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Variation in the fitness effects of mutations with population density and size in Escherichia coli.

Publication ,  Journal Article
Cao, H; Butler, K; Hossain, M; Lewis, JD
Published in: PloS one
January 2014

The fitness effects of mutations are context specific and depend on both external (e.g., environment) and internal (e.g., cellular stress, genetic background) factors. The influence of population size and density on fitness effects are unknown, despite the central role population size plays in the supply and fixation of mutations. We addressed this issue by comparing the fitness of 92 Keio strains (Escherichia coli K12 single gene knockouts) at comparatively high (1.2×10(7) CFUs/mL) and low (2.5×10(2) CFUs/mL) densities, which also differed in population size (high: 1.2×10(8); low: 1.25×10(3)). Twenty-eight gene deletions (30%) exhibited a fitness difference, ranging from 5 to 174% (median: 35%), between the high and low densities. Our analyses suggest this variation among gene deletions in fitness responses reflected in part both gene orientation and function, of the gene properties we examined (genomic position, length, orientation, and function). Although we could not determine the relative effects of population density and size, our results suggest fitness effects of mutations vary with these two factors, and this variation is gene-specific. Besides being a mechanism for density-dependent selection (r-K selection), the dependence of fitness effects on population density and size has implications for any population that varies in size over time, including populations undergoing evolutionary rescue, species invasions into novel habitats, and cancer progression and metastasis. Further, combined with recent advances in understanding the roles of other context-specific factors in the fitness effects of mutations, our results will help address theoretical and applied biological questions more realistically.

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Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2014

Volume

9

Issue

8

Start / End Page

e105369

Related Subject Headings

  • Selection, Genetic
  • Mutation Rate
  • Mutation
  • Genetic Variation
  • General Science & Technology
  • Gene Knockout Techniques
  • Gene Deletion
  • Escherichia coli
  • Analysis of Variance
 

Citation

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Cao, H., Butler, K., Hossain, M., & Lewis, J. D. (2014). Variation in the fitness effects of mutations with population density and size in Escherichia coli. PloS One, 9(8), e105369. https://doi.org/10.1371/journal.pone.0105369
Cao, Huansheng, Kevin Butler, Mithi Hossain, and James D. Lewis. “Variation in the fitness effects of mutations with population density and size in Escherichia coli.PloS One 9, no. 8 (January 2014): e105369. https://doi.org/10.1371/journal.pone.0105369.
Cao H, Butler K, Hossain M, Lewis JD. Variation in the fitness effects of mutations with population density and size in Escherichia coli. PloS one. 2014 Jan;9(8):e105369.
Cao, Huansheng, et al. “Variation in the fitness effects of mutations with population density and size in Escherichia coli.PloS One, vol. 9, no. 8, Jan. 2014, p. e105369. Epmc, doi:10.1371/journal.pone.0105369.
Cao H, Butler K, Hossain M, Lewis JD. Variation in the fitness effects of mutations with population density and size in Escherichia coli. PloS one. 2014 Jan;9(8):e105369.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2014

Volume

9

Issue

8

Start / End Page

e105369

Related Subject Headings

  • Selection, Genetic
  • Mutation Rate
  • Mutation
  • Genetic Variation
  • General Science & Technology
  • Gene Knockout Techniques
  • Gene Deletion
  • Escherichia coli
  • Analysis of Variance