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Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples.

Publication ,  Journal Article
Dukes, PV; Strobbia, P; Ngo, HT; Odion, RA; Rocke, D; Lee, WT; Vo-Dinh, T
Published in: Anal Chim Acta
December 1, 2020

Developing countries have seen a rise in cancer incidence and are projected to harbor three-quarters of all cancer-related mortality by 2030. While disproportionally affected by the burden of cancer, these regions are ill-equipped to handle the diagnostic caseload. The low number of trained pathologists per capita results in delayed diagnosis and treatment, ultimately contributing to increased mortality rates. To address this issue, we developed a point-of-care (POC) plasmonic assay for direct detection of cancer as an alternative to pathological review. Whereas our assay has general applicability in many cancer diagnoses that involve tissue biopsies, we use head and neck cancer (HNC) as a model system because these tumors are increasingly prevalent in lower-income and underserved regions, due to risk factors such as smoking, drinking, and viral infection. Our method uses surface-enhanced Raman scattering (SERS) to detect unique RNA biomarkers from human biopsy samples without the need for complex target amplification machinery (e.g., PCR), making it time and resource-efficient. Unlike previous studies that required target amplification, this work represents a significant advance for HNC diagnosis directly in clinical samples, using only our SERS-based assay for RNA biomarkers. In this study, we tested our assay on 20 clinical samples, demonstrating the accuracy of the method in the diagnosis of head and neck squamous cell carcinoma. We reported sensitivity of 100% and specificity of 97%. Furthermore, we used a handheld Raman device to read the results in order to illustrate the applicability of our method for POC diagnosis of cancer in low-resource settings.

Duke Scholars

Published In

Anal Chim Acta

DOI

EISSN

1873-4324

Publication Date

December 1, 2020

Volume

1139

Start / End Page

111 / 118

Location

Netherlands

Related Subject Headings

  • Spectrum Analysis, Raman
  • Point-of-Care Systems
  • Neoplasms
  • Humans
  • Biomarkers, Tumor
  • Biological Assay
  • Analytical Chemistry
  • 4018 Nanotechnology
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
 

Citation

APA
Chicago
ICMJE
MLA
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Dukes, P. V., Strobbia, P., Ngo, H. T., Odion, R. A., Rocke, D., Lee, W. T., & Vo-Dinh, T. (2020). Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples. Anal Chim Acta, 1139, 111–118. https://doi.org/10.1016/j.aca.2020.09.003
Dukes, Priya V., Pietro Strobbia, Hoan T. Ngo, Ren A. Odion, Daniel Rocke, Walter T. Lee, and Tuan Vo-Dinh. “Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples.Anal Chim Acta 1139 (December 1, 2020): 111–18. https://doi.org/10.1016/j.aca.2020.09.003.
Dukes PV, Strobbia P, Ngo HT, Odion RA, Rocke D, Lee WT, et al. Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples. Anal Chim Acta. 2020 Dec 1;1139:111–8.
Dukes, Priya V., et al. “Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples.Anal Chim Acta, vol. 1139, Dec. 2020, pp. 111–18. Pubmed, doi:10.1016/j.aca.2020.09.003.
Dukes PV, Strobbia P, Ngo HT, Odion RA, Rocke D, Lee WT, Vo-Dinh T. Plasmonic assay for amplification-free cancer biomarkers detection in clinical tissue samples. Anal Chim Acta. 2020 Dec 1;1139:111–118.
Journal cover image

Published In

Anal Chim Acta

DOI

EISSN

1873-4324

Publication Date

December 1, 2020

Volume

1139

Start / End Page

111 / 118

Location

Netherlands

Related Subject Headings

  • Spectrum Analysis, Raman
  • Point-of-Care Systems
  • Neoplasms
  • Humans
  • Biomarkers, Tumor
  • Biological Assay
  • Analytical Chemistry
  • 4018 Nanotechnology
  • 4004 Chemical engineering
  • 3401 Analytical chemistry