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Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS.

Publication ,  Journal Article
Beasley, J; McCaw, P; Zhang, H; Young, SP; Stiles, AR
Published in: Clin Chim Acta
December 2020

PURPOSE: To develop a method for the combined analysis of plasma and serum glucosylsphingosine (lyso-Gb1) and globotriaosylsphingosine (lyso-Gb3), biomarkers of Gaucher disease (GD) and Fabry disease (FD), respectively. METHODS: Internal standards were added to 100 µL of plasma/serum and glycosphingolipids: lyso-Gb1, lyso-Gb3, and galactosylsphingosine (GalSph) were extracted with dichloromethane/methanol and analyzed by UPLC-MS/MS. Samples from unaffected controls and patients with GD were first analyzed using a HILIC column to separate lyso-Gb1 from its isomer, GalSph. Samples from patients with FD or GD were analyzed using a C18 column to measure lyso-Gb3 and the hexosylsphingosine (HexSph: lyso-Gb1 + GalSph) fraction in a single combined method. RESULTS: Extraction efficiency was between 73% and 87% and day-to-day variability showed a relative standard error of <7.5%. GalSph was determined to have minimal to no contribution to the HexSph fraction in samples from unaffected controls and patients with GD. Lyso-Gb3 and HexSph measurements by the combined method were in good agreement with established methods, with no bias. CONCLUSIONS: HexSph and lyso-Gb3 analysis by reversed-phase chromatography UPLC-MS/MS is a cost-effective, time-efficient approach for evaluating these glycosphingolipid biomarkers in patients with a suspected or confirmed diagnosis of GD and FD.

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Published In

Clin Chim Acta

DOI

EISSN

1873-3492

Publication Date

December 2020

Volume

511

Start / End Page

132 / 137

Location

Netherlands

Related Subject Headings

  • Tandem Mass Spectrometry
  • Sphingolipids
  • Psychosine
  • Humans
  • Glycolipids
  • General Clinical Medicine
  • Fabry Disease
  • Chromatography, Liquid
  • Chromatography, High Pressure Liquid
  • Biomarkers
 

Citation

APA
Chicago
ICMJE
MLA
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Beasley, J., McCaw, P., Zhang, H., Young, S. P., & Stiles, A. R. (2020). Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS. Clin Chim Acta, 511, 132–137. https://doi.org/10.1016/j.cca.2020.10.007
Beasley, James, Patricia McCaw, Haoyue Zhang, Sarah P. Young, and Ashlee R. Stiles. “Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS.Clin Chim Acta 511 (December 2020): 132–37. https://doi.org/10.1016/j.cca.2020.10.007.
Beasley J, McCaw P, Zhang H, Young SP, Stiles AR. Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS. Clin Chim Acta. 2020 Dec;511:132–7.
Beasley, James, et al. “Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS.Clin Chim Acta, vol. 511, Dec. 2020, pp. 132–37. Pubmed, doi:10.1016/j.cca.2020.10.007.
Beasley J, McCaw P, Zhang H, Young SP, Stiles AR. Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS. Clin Chim Acta. 2020 Dec;511:132–137.
Journal cover image

Published In

Clin Chim Acta

DOI

EISSN

1873-3492

Publication Date

December 2020

Volume

511

Start / End Page

132 / 137

Location

Netherlands

Related Subject Headings

  • Tandem Mass Spectrometry
  • Sphingolipids
  • Psychosine
  • Humans
  • Glycolipids
  • General Clinical Medicine
  • Fabry Disease
  • Chromatography, Liquid
  • Chromatography, High Pressure Liquid
  • Biomarkers