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Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters.

Publication ,  Journal Article
Park, EYH; Smith, MJ; Stropp, ES; Snapp, KR; DiVietro, JA; Walker, WF; Schmidtke, DW; Diamond, SL; Lawrence, MB
Published in: Biophysical journal
April 2002

A cell-scaled microbead system was used to analyze the force-dependent kinetics of P-selectin adhesive bonds independent of micromechanical properties of the neutrophil's surface microvilli, an elastic structure on which P-selectin ligand glycoprotein-1 (PSGL-1) is localized. Microvillus extension has been hypothesized in contributing to the dynamic range of leukocyte rolling observed in vivo during inflammatory processes. To evaluate PSGL-1/P-selectin bond kinetics of microbeads and neutrophils, rolling and tethering on P-selectin-coated substrates were compared in a parallel-plate flow chamber. The dissociation rates for PSGL-1 microbeads on P-selectin were briefer than those of neutrophils for any wall shear stress, and increased more rapidly with increasing flow. The microvillus length necessary to reconcile dissociation constants of PSGL-1 microbeads and neutrophils on P-selectin was 0.21 microm at 0.4 dyn/cm2, and increased to 1.58 microm at 2 dyn/cm2. The apparent elastic spring constant of the microvillus ranged from 1340 to 152 pN/microm at 0.4 and 2.0 dyn/cm2 wall shear stress. Scanning electron micrographs of neutrophils rolling on P-selectin confirmed the existence of micrometer-scaled tethers. Fixation of neutrophils to abrogate microvillus elasticity resulted in rolling behavior similar to PSGL-1 microbeads. Our results suggest that microvillus extension during transient PSGL-1/P-selectin bonding may enhance the robustness of neutrophil rolling interactions.

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Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2002

Volume

82

Issue

4

Start / End Page

1835 / 1847

Related Subject Headings

  • Time Factors
  • Stress, Mechanical
  • Protein Binding
  • Polystyrenes
  • P-Selectin
  • Neutrophils
  • Models, Statistical
  • Microvilli
  • Microspheres
  • Microscopy, Video
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Park, E. Y. H., Smith, M. J., Stropp, E. S., Snapp, K. R., DiVietro, J. A., Walker, W. F., … Lawrence, M. B. (2002). Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters. Biophysical Journal, 82(4), 1835–1847. https://doi.org/10.1016/s0006-3495(02)75534-3
Park, Eric Y. H., McRae J. Smith, Emily S. Stropp, Karen R. Snapp, Jeffrey A. DiVietro, William F. Walker, David W. Schmidtke, Scott L. Diamond, and Michael B. Lawrence. “Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters.Biophysical Journal 82, no. 4 (April 2002): 1835–47. https://doi.org/10.1016/s0006-3495(02)75534-3.
Park EYH, Smith MJ, Stropp ES, Snapp KR, DiVietro JA, Walker WF, et al. Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters. Biophysical journal. 2002 Apr;82(4):1835–47.
Park, Eric Y. H., et al. “Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters.Biophysical Journal, vol. 82, no. 4, Apr. 2002, pp. 1835–47. Epmc, doi:10.1016/s0006-3495(02)75534-3.
Park EYH, Smith MJ, Stropp ES, Snapp KR, DiVietro JA, Walker WF, Schmidtke DW, Diamond SL, Lawrence MB. Comparison of PSGL-1 microbead and neutrophil rolling: microvillus elongation stabilizes P-selectin bond clusters. Biophysical journal. 2002 Apr;82(4):1835–1847.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2002

Volume

82

Issue

4

Start / End Page

1835 / 1847

Related Subject Headings

  • Time Factors
  • Stress, Mechanical
  • Protein Binding
  • Polystyrenes
  • P-Selectin
  • Neutrophils
  • Models, Statistical
  • Microvilli
  • Microspheres
  • Microscopy, Video