Skip to main content
Journal cover image

Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation.

Publication ,  Journal Article
Ryu, B; Nagappan, S; Santos-Valencia, F; Lee, P; Rodriguez, E; Lackie, M; Takatoh, J; Franks, KM
Published in: Cell Rep
April 20, 2021

It is well established that seizures beget seizures, yet the cellular processes that underlie progressive epileptogenesis remain unclear. Here, we use optogenetics to briefly activate targeted populations of mouse piriform cortex (PCx) principal neurons in vivo. After just 3 or 4 days of stimulation, previously subconvulsive stimuli trigger massive, generalized seizures. Highly recurrent allocortices are especially prone to "optokindling." Optokindling upsets the balance of recurrent excitation and feedback inhibition. To understand how this balance is disrupted, we then selectively reactivate the same neurons in vitro. Surprisingly, we find no evidence of heterosynaptic potentiation; instead, we observe a marked, pathway-specific decrease in feedback inhibition. We find no loss of inhibitory interneurons; rather, decreased GABA synthesis in feedback inhibitory neurons appears to underlie weakened inhibition. Optokindling will allow precise identification of the molecular processes by which brain activity patterns can progressively and pathologically disrupt the balance of cortical excitation and inhibition.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

April 20, 2021

Volume

35

Issue

3

Start / End Page

109001

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Synaptic Transmission
  • Synapses
  • Stereotaxic Techniques
  • Seizures
  • Piriform Cortex
  • Optogenetics
  • Neurons
  • Mice
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ryu, B., Nagappan, S., Santos-Valencia, F., Lee, P., Rodriguez, E., Lackie, M., … Franks, K. M. (2021). Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation. Cell Rep, 35(3), 109001. https://doi.org/10.1016/j.celrep.2021.109001
Ryu, Brendan, Shivathmihai Nagappan, Fernando Santos-Valencia, Psyche Lee, Erica Rodriguez, Meredith Lackie, Jun Takatoh, and Kevin M. Franks. “Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation.Cell Rep 35, no. 3 (April 20, 2021): 109001. https://doi.org/10.1016/j.celrep.2021.109001.
Ryu B, Nagappan S, Santos-Valencia F, Lee P, Rodriguez E, Lackie M, et al. Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation. Cell Rep. 2021 Apr 20;35(3):109001.
Ryu, Brendan, et al. “Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation.Cell Rep, vol. 35, no. 3, Apr. 2021, p. 109001. Pubmed, doi:10.1016/j.celrep.2021.109001.
Ryu B, Nagappan S, Santos-Valencia F, Lee P, Rodriguez E, Lackie M, Takatoh J, Franks KM. Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation. Cell Rep. 2021 Apr 20;35(3):109001.
Journal cover image

Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

April 20, 2021

Volume

35

Issue

3

Start / End Page

109001

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Synaptic Transmission
  • Synapses
  • Stereotaxic Techniques
  • Seizures
  • Piriform Cortex
  • Optogenetics
  • Neurons
  • Mice
  • Male