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Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms

Publication ,  Journal Article
Carlsen, ED; Hay, C; Henard, CA; Popov, V; Garg, NJ; Soong, L
Published in: Infection and Immunity
November 2013

Neutrophils are the first cells to infiltrate to the site of promastigote infection, and these cells help to reduce parasite burden shortly after infection is initiated. Several clinical reports indicate that neutrophil recruitment is sustained over the course of leishmaniasis, and amastigote-laden neutrophils have been isolated from chronically infected patients and experimentally infected animals. The goal of this study was to compare how thioglycolate-elicited murine neutrophils respond to metacyclic promastigotes and amastigotes derived from axenic cultures or from the lesions of infected mice. Neutrophils efficiently internalized both amastigote and promastigote forms of the parasite, and phagocytosis was enhanced in lipopolysaccharide (LPS)-activated neutrophils or when parasites were opsonized in serum from infected mice. Parasite uptake resulted in neutrophil activation, oxidative burst, and accelerated neutrophil death. While promastigotes triggered the release of tumor necrosis factor alpha (TNF-α), uptake of amastigotes preferentially resulted in the secretion of interleukin-10 (IL-10) from neutrophils. Finally, the majority of promastigotes were killed by neutrophils, while axenic culture- and lesion-derived amastigotes were highly resistant to neutrophil microbicidal mechanisms. This study indicates that neutrophils exhibit distinct responses to promastigote and amastigote infection. Our findings have important implications for determining the impact of sustained neutrophil recruitment and amastigote-neutrophil interactions during the late phase of cutaneous leishmaniasis.

Duke Scholars

Published In

Infection and Immunity

DOI

EISSN

1098-5522

ISSN

0019-9567

Publication Date

November 2013

Volume

81

Issue

11

Start / End Page

3966 / 3974

Publisher

American Society for Microbiology

Related Subject Headings

  • Microbiology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Carlsen, E. D., Hay, C., Henard, C. A., Popov, V., Garg, N. J., & Soong, L. (2013). Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms. Infection and Immunity, 81(11), 3966–3974. https://doi.org/10.1128/iai.00770-13
Carlsen, Eric D., Christie Hay, Calvin A. Henard, Vsevolod Popov, Nisha Jain Garg, and Lynn Soong. “Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms.” Edited by J. L. Flynn. Infection and Immunity 81, no. 11 (November 2013): 3966–74. https://doi.org/10.1128/iai.00770-13.
Carlsen ED, Hay C, Henard CA, Popov V, Garg NJ, Soong L. Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms. Flynn JL, editor. Infection and Immunity. 2013 Nov;81(11):3966–74.
Carlsen, Eric D., et al. “Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms.” Infection and Immunity, edited by J. L. Flynn, vol. 81, no. 11, American Society for Microbiology, Nov. 2013, pp. 3966–74. Crossref, doi:10.1128/iai.00770-13.
Carlsen ED, Hay C, Henard CA, Popov V, Garg NJ, Soong L. Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms. Flynn JL, editor. Infection and Immunity. American Society for Microbiology; 2013 Nov;81(11):3966–3974.

Published In

Infection and Immunity

DOI

EISSN

1098-5522

ISSN

0019-9567

Publication Date

November 2013

Volume

81

Issue

11

Start / End Page

3966 / 3974

Publisher

American Society for Microbiology

Related Subject Headings

  • Microbiology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences