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Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome.

Publication ,  Journal Article
Jayasundara, N; Gardner, LD; Block, BA
Published in: American journal of physiology. Regulatory, integrative and comparative physiology
November 2013

Little is known about the mechanisms underpinning thermal plasticity of vertebrate hearts. Bluefin tuna hearts offer a unique model to investigate processes underlying thermal acclimation. Their hearts, while supporting an endothermic physiology, operate at ambient temperature, and are presented with a thermal challenge when migrating to different thermal regimes. Here, we examined the molecular responses in atrial and ventricular tissues of Pacific bluefin tuna acclimated to 14°C, 20°C, and 25°C. Quantitative PCR studies showed an increase in sarcoplasmic reticulum Ca(2+) ATPase gene expression with cold acclimation and an induction of Na(+)/Ca(2+)-exchanger gene at both cold and warm temperatures. These data provide evidence for thermal plasticity of excitation-contraction coupling gene expression in bluefin tunas and indicate an increased capacity for internal Ca(2+) storage in cardiac myocytes at 14°C. Transcriptomic analysis showed profound changes in cardiac tissues with acclimation. A principal component analysis revealed that temperature effect was greatest on gene expression in warm-acclimated atrium. Overall data showed an increase in cardiac energy metabolism at 14°C, potentially compensating for cold temperature to optimize bluefin tuna performance in colder oceans. In contrast, metabolic enzyme activity and gene expression data suggest a decrease in ATP production at 25°C. Expression of genes involved in protein turnover and molecular chaperones was also decreased at 25°C. Expression of genes involved in oxidative stress response and programmed cell death suggest an increase in oxidative damage and apoptosis at 25°C, particularly in the atrium. These findings provide insights into molecular processes that may characterize cardiac phenotypes at upper thermal limits of teleosts.

Duke Scholars

Published In

American journal of physiology. Regulatory, integrative and comparative physiology

DOI

EISSN

1522-1490

ISSN

0363-6119

Publication Date

November 2013

Volume

305

Issue

9

Start / End Page

R1010 / R1020

Related Subject Headings

  • Tuna
  • Temperature
  • Sodium-Calcium Exchanger
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Ryanodine Receptor Calcium Release Channel
  • Principal Component Analysis
  • Polymerase Chain Reaction
  • Physiology
  • Oxidative Stress
  • Oligonucleotide Array Sequence Analysis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jayasundara, N., Gardner, L. D., & Block, B. A. (2013). Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 305(9), R1010–R1020. https://doi.org/10.1152/ajpregu.00254.2013
Jayasundara, Nishad, Luke D. Gardner, and Barbara A. Block. “Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome.American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 305, no. 9 (November 2013): R1010–20. https://doi.org/10.1152/ajpregu.00254.2013.
Jayasundara N, Gardner LD, Block BA. Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome. American journal of physiology Regulatory, integrative and comparative physiology. 2013 Nov;305(9):R1010–20.
Jayasundara, Nishad, et al. “Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome.American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 305, no. 9, Nov. 2013, pp. R1010–20. Epmc, doi:10.1152/ajpregu.00254.2013.
Jayasundara N, Gardner LD, Block BA. Effects of temperature acclimation on Pacific bluefin tuna (Thunnus orientalis) cardiac transcriptome. American journal of physiology Regulatory, integrative and comparative physiology. 2013 Nov;305(9):R1010–R1020.

Published In

American journal of physiology. Regulatory, integrative and comparative physiology

DOI

EISSN

1522-1490

ISSN

0363-6119

Publication Date

November 2013

Volume

305

Issue

9

Start / End Page

R1010 / R1020

Related Subject Headings

  • Tuna
  • Temperature
  • Sodium-Calcium Exchanger
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Ryanodine Receptor Calcium Release Channel
  • Principal Component Analysis
  • Polymerase Chain Reaction
  • Physiology
  • Oxidative Stress
  • Oligonucleotide Array Sequence Analysis