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Anti-lipopolysaccharide factors in the American lobster Homarus americanus: molecular characterization and transcriptional response to Vibrio fluvialis challenge.

Publication ,  Journal Article
Beale, KM; Towle, DW; Jayasundara, N; Smith, CM; Shields, JD; Small, HJ; Greenwood, SJ
Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics
December 2008

Two partial mRNA sequences predicted to encode anti-lipopolysaccharide factors (ALFs) were identified among expressed sequence tags generated from the American lobster Homarus americanus and complete cDNA sequences were obtained from library clones. Comparison of the translated amino acid sequences to those publicly available confirmed similarity to arthropod anti-lipopolysaccharide factors. Both protein sequences, designated ALFHa-1 and ALFHa-2, contained an N-terminal signal peptide and two half-cysteines participating in a disulfide bridge, features conserved in other ALFs. Predicted secondary structures were similar to that described for the ALF from the horseshoe crab Limulus polyphemus. As part of an exploratory study of immunity in H. americanus, lobsters were injected with the bacterium Vibrio fluvialis and gill, hematopoietic, and hepatopancreas tissues were sampled for analysis of gene expression of ALFHa-1 and ALFHa-2 by quantitative PCR. The relative abundance of ALFHa-2 mRNA was not significantly affected by Vibrio injection in any of the three tissues tested. In contrast, ALFHa-1 mRNA levels in gills were increased by the treatment some 17-fold. Our results support a molecularly specific regulation of antimicrobial proteins in response to bacterial infection in H. americanus.

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Published In

Comparative biochemistry and physiology. Part D, Genomics & proteomics

DOI

EISSN

1878-0407

ISSN

1744-117X

Publication Date

December 2008

Volume

3

Issue

4

Start / End Page

263 / 269

Related Subject Headings

  • Genetics & Heredity
  • 3105 Genetics
  • 0604 Genetics
 

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Beale, K. M., Towle, D. W., Jayasundara, N., Smith, C. M., Shields, J. D., Small, H. J., & Greenwood, S. J. (2008). Anti-lipopolysaccharide factors in the American lobster Homarus americanus: molecular characterization and transcriptional response to Vibrio fluvialis challenge. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 3(4), 263–269. https://doi.org/10.1016/j.cbd.2008.07.001
Beale, K. M., D. W. Towle, N. Jayasundara, C. M. Smith, J. D. Shields, H. J. Small, and S. J. Greenwood. “Anti-lipopolysaccharide factors in the American lobster Homarus americanus: molecular characterization and transcriptional response to Vibrio fluvialis challenge.Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics 3, no. 4 (December 2008): 263–69. https://doi.org/10.1016/j.cbd.2008.07.001.
Beale KM, Towle DW, Jayasundara N, Smith CM, Shields JD, Small HJ, et al. Anti-lipopolysaccharide factors in the American lobster Homarus americanus: molecular characterization and transcriptional response to Vibrio fluvialis challenge. Comparative biochemistry and physiology Part D, Genomics & proteomics. 2008 Dec;3(4):263–9.
Beale, K. M., et al. “Anti-lipopolysaccharide factors in the American lobster Homarus americanus: molecular characterization and transcriptional response to Vibrio fluvialis challenge.Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, vol. 3, no. 4, Dec. 2008, pp. 263–69. Epmc, doi:10.1016/j.cbd.2008.07.001.
Beale KM, Towle DW, Jayasundara N, Smith CM, Shields JD, Small HJ, Greenwood SJ. Anti-lipopolysaccharide factors in the American lobster Homarus americanus: molecular characterization and transcriptional response to Vibrio fluvialis challenge. Comparative biochemistry and physiology Part D, Genomics & proteomics. 2008 Dec;3(4):263–269.
Journal cover image

Published In

Comparative biochemistry and physiology. Part D, Genomics & proteomics

DOI

EISSN

1878-0407

ISSN

1744-117X

Publication Date

December 2008

Volume

3

Issue

4

Start / End Page

263 / 269

Related Subject Headings

  • Genetics & Heredity
  • 3105 Genetics
  • 0604 Genetics