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Dietary omega-3 fatty acid intake impacts peripheral blood DNA methylation -anti-inflammatory effects and individual variability in a pilot study.

Publication ,  Journal Article
Frankhouser, DE; Steck, S; Sovic, MG; Belury, MA; Wang, Q; Clinton, SK; Bundschuh, R; Yan, PS; Yee, LD
Published in: J Nutr Biochem
January 2022

Omega-3 or n-3 polyunsaturated fatty acids (PUFAs) are widely studied for health benefits that may relate to anti-inflammatory activity. However, mechanisms mediating an anti-inflammatory response to n-3 PUFA intake are not fully understood. Of interest is the emerging role of fatty acids to impact DNA methylation (DNAm) and thereby modulate mediating inflammatory processes. In this pilot study, we investigated the impact of n-3 PUFA intake on DNAm in inflammation-related signaling pathways in peripheral blood mononuclear cells (PBMCs) of women at high risk of breast cancer. PBMCs of women at high risk of breast cancer (n=10) were obtained at baseline and after 6 months of n-3 PUFA (5 g/d EPA+DHA dose arm) intake in a previously reported dose finding trial. DNA methylation of PBMCs was assayed by reduced representation bisulfite sequencing (RRBS) to obtain genome-wide methylation profiles at the single nucleotide level. We examined the impact of n-3 PUFA on genome-wide DNAm and focused upon a set of candidate genes associated with inflammation signaling pathways and breast cancer. We identified 24,842 differentially methylated CpGs (DMCs) in gene promoters of 5507 genes showing significant enrichment for hypermethylation in both the candidate gene and genome-wide analyses. Pathway analysis identified significantly hypermethylated signaling networks after n-3 PUFA treatment, such as the Toll-like Receptor inflammatory pathway. The DNAm pattern in individuals and the response to n-3 PUFA intake are heterogeneous. PBMC DNAm profiling suggests a mechanism whereby n-3 PUFAs may impact inflammatory cascades associated with disease processes including carcinogenesis.

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Published In

J Nutr Biochem

DOI

EISSN

1873-4847

Publication Date

January 2022

Volume

99

Start / End Page

108839

Location

United States

Related Subject Headings

  • Toll-Like Receptors
  • Pilot Projects
  • Nutrition & Dietetics
  • Middle Aged
  • Leukocytes, Mononuclear
  • Humans
  • Female
  • Fatty Acids, Omega-3
  • Dietary Supplements
  • DNA Methylation
 

Citation

APA
Chicago
ICMJE
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Frankhouser, D. E., Steck, S., Sovic, M. G., Belury, M. A., Wang, Q., Clinton, S. K., … Yee, L. D. (2022). Dietary omega-3 fatty acid intake impacts peripheral blood DNA methylation -anti-inflammatory effects and individual variability in a pilot study. J Nutr Biochem, 99, 108839. https://doi.org/10.1016/j.jnutbio.2021.108839
Frankhouser, David E., Sarah Steck, Michael G. Sovic, Martha A. Belury, Qianben Wang, Steven K. Clinton, Ralf Bundschuh, Pearlly S. Yan, and Lisa D. Yee. “Dietary omega-3 fatty acid intake impacts peripheral blood DNA methylation -anti-inflammatory effects and individual variability in a pilot study.J Nutr Biochem 99 (January 2022): 108839. https://doi.org/10.1016/j.jnutbio.2021.108839.
Frankhouser DE, Steck S, Sovic MG, Belury MA, Wang Q, Clinton SK, et al. Dietary omega-3 fatty acid intake impacts peripheral blood DNA methylation -anti-inflammatory effects and individual variability in a pilot study. J Nutr Biochem. 2022 Jan;99:108839.
Frankhouser, David E., et al. “Dietary omega-3 fatty acid intake impacts peripheral blood DNA methylation -anti-inflammatory effects and individual variability in a pilot study.J Nutr Biochem, vol. 99, Jan. 2022, p. 108839. Pubmed, doi:10.1016/j.jnutbio.2021.108839.
Frankhouser DE, Steck S, Sovic MG, Belury MA, Wang Q, Clinton SK, Bundschuh R, Yan PS, Yee LD. Dietary omega-3 fatty acid intake impacts peripheral blood DNA methylation -anti-inflammatory effects and individual variability in a pilot study. J Nutr Biochem. 2022 Jan;99:108839.
Journal cover image

Published In

J Nutr Biochem

DOI

EISSN

1873-4847

Publication Date

January 2022

Volume

99

Start / End Page

108839

Location

United States

Related Subject Headings

  • Toll-Like Receptors
  • Pilot Projects
  • Nutrition & Dietetics
  • Middle Aged
  • Leukocytes, Mononuclear
  • Humans
  • Female
  • Fatty Acids, Omega-3
  • Dietary Supplements
  • DNA Methylation