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Biomimetic polydopamine-laced hydroxyapatite collagen material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts.

Publication ,  Journal Article
Wang, L; Wu, T-H; Hu, X; Liu, J; Wu, D; Miguez, PA; Wright, JT; Zhang, S; Chi, J-T; Tseng, HC; Ko, C-C
Published in: Biomater Sci
November 9, 2021

Polydopamine-assisted modification for bone substitute materials has recently shown great application potential in bone tissue engineering due to its excellent biocompatibility and adhesive properties. A scaffold material's impact on osteoclasts is equally as important as its impact on osteoblasts when considering tissue engineering for bone defect repair, as healthy bone regeneration requires an orchestrated coupling between osteoclasts and osteoblasts. How polydopamine-functionalized bone substitute materials modulate the activity of osteoblast lineage cells has been extensively investigated, but much less is known about their impact on osteoclasts. Moreover, most of the polydopamine-functionalized materials would need to additionally load a biomolecule to exert the modulation on osteoclast activity. Herein, we demonstrated that our biomimetic polydopamine-laced hydroxyapatite collagen (PDHC) scaffold material, which does not need to load additional bioactive agent, is sufficiently able to modulate osteoclast activity in vitro. First, PDHC showed an anti-resorptive potential, characterized by decreased osteoclast differentiation and resorption capacity and changes in osteoclasts' transcriptome profile. Next, cAMP response element-binding protein (CREB) activity was found to mediate PDHC's anti-osteoclastogenic effect. Finally, although PDHC altered clastokines expression pattern of osteoclasts, as revealed by transcriptomic and secretomic analysis, osteoclasts' coupling to osteoblasts was not compromised by PDHC. Collectively, this study demonstrated the PDHC material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts. Such a feature is favorable for the net increase of bone mass, which endows the PDHC material with great application potential in preclinical/clinical bone defect repair.

Duke Scholars

Published In

Biomater Sci

DOI

EISSN

2047-4849

Publication Date

November 9, 2021

Volume

9

Issue

22

Start / End Page

7565 / 7574

Location

England

Related Subject Headings

  • Polymers
  • Osteoclasts
  • Osteoblasts
  • Indoles
  • Humans
  • Durapatite
  • Collagen
  • Cell Differentiation
  • Bone Resorption
  • Biomimetics
 

Citation

APA
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ICMJE
MLA
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Wang, L., Wu, T.-H., Hu, X., Liu, J., Wu, D., Miguez, P. A., … Ko, C.-C. (2021). Biomimetic polydopamine-laced hydroxyapatite collagen material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts. Biomater Sci, 9(22), 7565–7574. https://doi.org/10.1039/d1bm01119g
Wang, Lufei, Tai-Hsien Wu, Xiangxiang Hu, Jie Liu, Di Wu, Patricia A. Miguez, John Timothy Wright, et al. “Biomimetic polydopamine-laced hydroxyapatite collagen material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts.Biomater Sci 9, no. 22 (November 9, 2021): 7565–74. https://doi.org/10.1039/d1bm01119g.
Wang L, Wu T-H, Hu X, Liu J, Wu D, Miguez PA, Wright JT, Zhang S, Chi J-T, Tseng HC, Ko C-C. Biomimetic polydopamine-laced hydroxyapatite collagen material orients osteoclast behavior to an anti-resorptive pattern without compromising osteoclasts' coupling to osteoblasts. Biomater Sci. 2021 Nov 9;9(22):7565–7574.
Journal cover image

Published In

Biomater Sci

DOI

EISSN

2047-4849

Publication Date

November 9, 2021

Volume

9

Issue

22

Start / End Page

7565 / 7574

Location

England

Related Subject Headings

  • Polymers
  • Osteoclasts
  • Osteoblasts
  • Indoles
  • Humans
  • Durapatite
  • Collagen
  • Cell Differentiation
  • Bone Resorption
  • Biomimetics