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Glucocorticoid-Responsive Transcription Factor Krüppel-Like Factor 9 Regulates fkbp5 and Metabolism.

Publication ,  Journal Article
Gans, IM; Grendler, J; Babich, R; Jayasundara, N; Coffman, JA
Published in: Frontiers in cell and developmental biology
January 2021

Krüppel-like factor 9 (Klf9) is a feedforward regulator of glucocorticoid receptor (GR) signaling. Here we show that in zebrafish klf9 is expressed with GR-dependent oscillatory dynamics in synchrony with fkbp5, a GR target that encodes a negative feedback regulator of GR signaling. We found that fkbp5 transcript levels are elevated in klf9-/- mutants and that Klf9 associates with chromatin at the fkbp5 promoter, which becomes hyperacetylated in klf9-/- mutants, suggesting that the GR regulates fkbp5 via an incoherent feedforward loop with klf9. As both the GR and Fkbp5 are known to regulate metabolism, we asked how loss of Klf9 affects metabolic rate and gene expression. We found that klf9-/- mutants have a decreased oxygen consumption rate (OCR) and upregulate glycolytic genes, the promoter regions of which are enriched for potential Klf9 binding motifs. Our results suggest that Klf9 functions downstream of the GR to regulate cellular glucocorticoid responsivity and metabolic homeostasis.

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Published In

Frontiers in cell and developmental biology

DOI

EISSN

2296-634X

ISSN

2296-634X

Publication Date

January 2021

Volume

9

Start / End Page

727037

Related Subject Headings

  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

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Gans, I. M., Grendler, J., Babich, R., Jayasundara, N., & Coffman, J. A. (2021). Glucocorticoid-Responsive Transcription Factor Krüppel-Like Factor 9 Regulates fkbp5 and Metabolism. Frontiers in Cell and Developmental Biology, 9, 727037. https://doi.org/10.3389/fcell.2021.727037
Gans, Ian M., Janelle Grendler, Remy Babich, Nishad Jayasundara, and James A. Coffman. “Glucocorticoid-Responsive Transcription Factor Krüppel-Like Factor 9 Regulates fkbp5 and Metabolism.Frontiers in Cell and Developmental Biology 9 (January 2021): 727037. https://doi.org/10.3389/fcell.2021.727037.
Gans IM, Grendler J, Babich R, Jayasundara N, Coffman JA. Glucocorticoid-Responsive Transcription Factor Krüppel-Like Factor 9 Regulates fkbp5 and Metabolism. Frontiers in cell and developmental biology. 2021 Jan;9:727037.
Gans, Ian M., et al. “Glucocorticoid-Responsive Transcription Factor Krüppel-Like Factor 9 Regulates fkbp5 and Metabolism.Frontiers in Cell and Developmental Biology, vol. 9, Jan. 2021, p. 727037. Epmc, doi:10.3389/fcell.2021.727037.
Gans IM, Grendler J, Babich R, Jayasundara N, Coffman JA. Glucocorticoid-Responsive Transcription Factor Krüppel-Like Factor 9 Regulates fkbp5 and Metabolism. Frontiers in cell and developmental biology. 2021 Jan;9:727037.

Published In

Frontiers in cell and developmental biology

DOI

EISSN

2296-634X

ISSN

2296-634X

Publication Date

January 2021

Volume

9

Start / End Page

727037

Related Subject Headings

  • 32 Biomedical and clinical sciences
  • 31 Biological sciences