Skip to main content
Journal cover image

FEF inactivation with improved optogenetic methods.

Publication ,  Journal Article
Acker, L; Pino, EN; Boyden, ES; Desimone, R
Published in: Proc Natl Acad Sci U S A
November 15, 2016

Optogenetic methods have been highly effective for suppressing neural activity and modulating behavior in rodents, but effects have been much smaller in primates, which have much larger brains. Here, we present a suite of technologies to use optogenetics effectively in primates and apply these tools to a classic question in oculomotor control. First, we measured light absorption and heat propagation in vivo, optimized the conditions for using the red-light-shifted halorhodopsin Jaws in primates, and developed a large-volume illuminator to maximize light delivery with minimal heating and tissue displacement. Together, these advances allowed for nearly universal neuronal inactivation across more than 10 mm3 of the cortex. Using these tools, we demonstrated large behavioral changes (i.e., up to several fold increases in error rate) with relatively low light power densities (≤100 mW/mm2) in the frontal eye field (FEF). Pharmacological inactivation studies have shown that the FEF is critical for executing saccades to remembered locations. FEF neurons increase their firing rate during the three epochs of the memory-guided saccade task: visual stimulus presentation, the delay interval, and motor preparation. It is unclear from earlier work, however, whether FEF activity during each epoch is necessary for memory-guided saccade execution. By harnessing the temporal specificity of optogenetics, we found that FEF contributes to memory-guided eye movements during every epoch of the memory-guided saccade task (the visual, delay, and motor periods).

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

November 15, 2016

Volume

113

Issue

46

Start / End Page

E7297 / E7306

Location

United States

Related Subject Headings

  • Saccades
  • Photic Stimulation
  • Optogenetics
  • Neurons
  • Mice, Inbred C57BL
  • Memory
  • Male
  • Macaca mulatta
  • Hot Temperature
  • Halorhodopsins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Acker, L., Pino, E. N., Boyden, E. S., & Desimone, R. (2016). FEF inactivation with improved optogenetic methods. Proc Natl Acad Sci U S A, 113(46), E7297–E7306. https://doi.org/10.1073/pnas.1610784113
Acker, Leah, Erica N. Pino, Edward S. Boyden, and Robert Desimone. “FEF inactivation with improved optogenetic methods.Proc Natl Acad Sci U S A 113, no. 46 (November 15, 2016): E7297–7306. https://doi.org/10.1073/pnas.1610784113.
Acker L, Pino EN, Boyden ES, Desimone R. FEF inactivation with improved optogenetic methods. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7297–306.
Acker, Leah, et al. “FEF inactivation with improved optogenetic methods.Proc Natl Acad Sci U S A, vol. 113, no. 46, Nov. 2016, pp. E7297–306. Pubmed, doi:10.1073/pnas.1610784113.
Acker L, Pino EN, Boyden ES, Desimone R. FEF inactivation with improved optogenetic methods. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7297–E7306.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

November 15, 2016

Volume

113

Issue

46

Start / End Page

E7297 / E7306

Location

United States

Related Subject Headings

  • Saccades
  • Photic Stimulation
  • Optogenetics
  • Neurons
  • Mice, Inbred C57BL
  • Memory
  • Male
  • Macaca mulatta
  • Hot Temperature
  • Halorhodopsins