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Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system.

Publication ,  Chapter
Baker, LG; Lodge, JK
2012

Reverse genetics is commonly used to identify and characterize genes involved in a variety of cellular processes. There is a limited set of positive selectable markers available for use in making gene deletions or other genetic manipulations in Cryptococcus neoformans. Here, we describe the adaptation of the Bacteriophage P1 Cre-loxP system for use in C. neoformans, and its application in the excision and reuse of the geneticin drug marker. This tool will allow investigators to make multiple, sequential gene deletions in the same strain, which should facilitate the analysis of multigene families.

Duke Scholars

DOI

Publication Date

2012

Volume

845

Start / End Page

85 / 98

Related Subject Headings

  • Mutagenesis, Site-Directed
  • Integrases
  • Gentamicins
  • Genetic Markers
  • Gene Deletion
  • Developmental Biology
  • Cryptococcus neoformans
  • Bacteriophage P1
  • Attachment Sites, Microbiological
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Baker, L. G., & Lodge, J. K. (2012). Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system. (Vol. 845, pp. 85–98). https://doi.org/10.1007/978-1-61779-539-8_6
Baker, Lorina G., and Jennifer K. Lodge. “Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system.,” 845:85–98, 2012. https://doi.org/10.1007/978-1-61779-539-8_6.
Baker, Lorina G., and Jennifer K. Lodge. Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system. Vol. 845, 2012, pp. 85–98. Pubmed, doi:10.1007/978-1-61779-539-8_6.

DOI

Publication Date

2012

Volume

845

Start / End Page

85 / 98

Related Subject Headings

  • Mutagenesis, Site-Directed
  • Integrases
  • Gentamicins
  • Genetic Markers
  • Gene Deletion
  • Developmental Biology
  • Cryptococcus neoformans
  • Bacteriophage P1
  • Attachment Sites, Microbiological
  • 0601 Biochemistry and Cell Biology