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Three galactose inducible promoters for use in C. neoformans var. grubii.

Publication ,  Journal Article
Ruff, JA; Lodge, JK; Baker, LG
Published in: Fungal Genet Biol
January 2009
Published version (DOI) Link to item

Cryptococcus neoformans is the causative agent of cryptococcal meningoencephalitis, most frequently occurring in immunocompromised individuals. There are three varieties of C. neoformans, var. grubii, var. neoformans, and var. gatti. Worldwide var. grubii is the most prevalent clinical isolate. However, few tools for the study of essential genes in var. grubii exist. Here we describe three endogenous inducible promoters for use in the study of this important opportunistic pathogen. We identified eight potential homologs of S. cerevisiae galactose genes in var. grubii. We found that GAL1, GAL7, and UGE2 were regulated by glucose and galactose and can be used successfully during mating. Our analysis indicated these promoters should prove to be excellent tools for analysis of genes in var. grubii.

Duke Scholars

Jennifer Lodge
Author Jennifer Lodge Molecular Genetics and Microbiology

Published In

Fungal Genet Biol

DOI

10.1016/j.fgb.2008.10.003

EISSN

1096-0937

Publication Date

January 2009

Volume

46

Issue

1

Start / End Page

9 / 16

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Promoter Regions, Genetic
  • Microbiology
  • Genes, Fungal
  • Gene Expression Regulation, Fungal
  • Gene Expression Profiling
  • Galactose
  • Cryptococcus neoformans
  • 0607 Plant Biology
  • 0605 Microbiology
 

Citation

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ICMJE
MLA
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Ruff, J. A., Lodge, J. K., & Baker, L. G. (2009). Three galactose inducible promoters for use in C. neoformans var. grubii. Fungal Genet Biol, 46(1), 9–16. https://doi.org/10.1016/j.fgb.2008.10.003
Ruff, Jack A., Jennifer K. Lodge, and Lorina G. Baker. “Three galactose inducible promoters for use in C. neoformans var. grubii.” Fungal Genet Biol 46, no. 1 (January 2009): 9–16. https://doi.org/10.1016/j.fgb.2008.10.003.
Ruff JA, Lodge JK, Baker LG. Three galactose inducible promoters for use in C. neoformans var. grubii. Fungal Genet Biol. 2009 Jan;46(1):9–16.
Ruff, Jack A., et al. “Three galactose inducible promoters for use in C. neoformans var. grubii.” Fungal Genet Biol, vol. 46, no. 1, Jan. 2009, pp. 9–16. Pubmed, doi:10.1016/j.fgb.2008.10.003.
Ruff JA, Lodge JK, Baker LG. Three galactose inducible promoters for use in C. neoformans var. grubii. Fungal Genet Biol. 2009 Jan;46(1):9–16.
Journal cover image

Published In

Fungal Genet Biol

DOI

10.1016/j.fgb.2008.10.003

EISSN

1096-0937

Publication Date

January 2009

Volume

46

Issue

1

Start / End Page

9 / 16

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Promoter Regions, Genetic
  • Microbiology
  • Genes, Fungal
  • Gene Expression Regulation, Fungal
  • Gene Expression Profiling
  • Galactose
  • Cryptococcus neoformans
  • 0607 Plant Biology
  • 0605 Microbiology