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Transposon Tn5 target specificity: preference for insertion at G/C pairs.

Publication ,  Journal Article
Lodge, JK; Weston-Hafer, K; Berg, DE
Published in: Genetics
November 1988

The procaryotic transposon Tn5 inserts into many different sites within a single gene, but some sites (hotspots) are targeted repeatedly. Hotspots are not closely related in sequence, but most have G/C pairs at the ends of the nine base pairs duplicated by Tn5 insertion. In pBR322, the major hotspot coincides with the "-10 region" of the tet promoter. We mutated the G/C pairs at this hotspot and assayed for insertion into hotspot I, resistance to tetracycline, and plasmid supercoiling. We found that changing the G/C pairs to A/T pairs reduced the frequency of insertion into the hotspot by at least fivefold. The reduction in hotspot use caused by these G/C to A/T changes was not attributable to changes in plasmid supercoiling or tet promoter strength.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

November 1988

Volume

120

Issue

3

Start / End Page

645 / 650

Location

United States

Related Subject Headings

  • Tetracycline Resistance
  • Plasmids
  • Mutation
  • Guanine
  • Escherichia coli
  • Developmental Biology
  • DNA, Superhelical
  • DNA Transposable Elements
  • Cytosine
  • Base Composition
 

Citation

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Lodge, J. K., Weston-Hafer, K., & Berg, D. E. (1988). Transposon Tn5 target specificity: preference for insertion at G/C pairs. Genetics, 120(3), 645–650. https://doi.org/10.1093/genetics/120.3.645
Lodge, J. K., K. Weston-Hafer, and D. E. Berg. “Transposon Tn5 target specificity: preference for insertion at G/C pairs.Genetics 120, no. 3 (November 1988): 645–50. https://doi.org/10.1093/genetics/120.3.645.
Lodge JK, Weston-Hafer K, Berg DE. Transposon Tn5 target specificity: preference for insertion at G/C pairs. Genetics. 1988 Nov;120(3):645–50.
Lodge, J. K., et al. “Transposon Tn5 target specificity: preference for insertion at G/C pairs.Genetics, vol. 120, no. 3, Nov. 1988, pp. 645–50. Pubmed, doi:10.1093/genetics/120.3.645.
Lodge JK, Weston-Hafer K, Berg DE. Transposon Tn5 target specificity: preference for insertion at G/C pairs. Genetics. 1988 Nov;120(3):645–650.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

November 1988

Volume

120

Issue

3

Start / End Page

645 / 650

Location

United States

Related Subject Headings

  • Tetracycline Resistance
  • Plasmids
  • Mutation
  • Guanine
  • Escherichia coli
  • Developmental Biology
  • DNA, Superhelical
  • DNA Transposable Elements
  • Cytosine
  • Base Composition