Skip to main content

Posttranslational, translational, and transcriptional responses to nitric oxide stress in Cryptococcus neoformans: implications for virulence.

Publication ,  Journal Article
Missall, TA; Pusateri, ME; Donlin, MJ; Chambers, KT; Corbett, JA; Lodge, JK
Published in: Eukaryot Cell
March 2006

The ability of the fungal pathogen Cryptococcus neoformans to evade the mammalian innate immune response and cause disease is partially due to its ability to respond to and survive nitrosative stress. In this study, we use proteomic and genomic approaches to elucidate the response of C. neoformans to nitric oxide stress. This nitrosative stress response involves both transcriptional, translational, and posttranslational regulation. Proteomic and genomic analyses reveal changes in expression of stress response genes. In addition, genes involved in cell wall organization, respiration, signal transduction, transport, transcriptional control, and metabolism show altered expression under nitrosative conditions. Posttranslational modifications of transaldolase (Tal1), aconitase (Aco1), and the thiol peroxidase, Tsa1, are regulated during nitrosative stress. One stress-related protein up-regulated in the presence of nitric oxide stress is glutathione reductase (Glr1). To further investigate its functional role during nitrosative stress, a deletion mutant was generated. We show that this glr1Delta mutant is sensitive to nitrosative stress and macrophage killing in addition to being avirulent in mice. These studies define the response to nitrosative stress in this important fungal pathogen.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

March 2006

Volume

5

Issue

3

Start / End Page

518 / 529

Location

United States

Related Subject Headings

  • Virulence
  • Transcription, Genetic
  • Transaldolase
  • Proteomics
  • Protein Processing, Post-Translational
  • Peroxidases
  • Oxidative Stress
  • Nitric Oxide
  • Microbiology
  • Mice, Inbred CBA
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Missall, T. A., Pusateri, M. E., Donlin, M. J., Chambers, K. T., Corbett, J. A., & Lodge, J. K. (2006). Posttranslational, translational, and transcriptional responses to nitric oxide stress in Cryptococcus neoformans: implications for virulence. Eukaryot Cell, 5(3), 518–529. https://doi.org/10.1128/EC.5.3.518-529.2006
Missall, Tricia A., Mary Ellen Pusateri, Maureen J. Donlin, Kari T. Chambers, John A. Corbett, and Jennifer K. Lodge. “Posttranslational, translational, and transcriptional responses to nitric oxide stress in Cryptococcus neoformans: implications for virulence.Eukaryot Cell 5, no. 3 (March 2006): 518–29. https://doi.org/10.1128/EC.5.3.518-529.2006.
Missall TA, Pusateri ME, Donlin MJ, Chambers KT, Corbett JA, Lodge JK. Posttranslational, translational, and transcriptional responses to nitric oxide stress in Cryptococcus neoformans: implications for virulence. Eukaryot Cell. 2006 Mar;5(3):518–29.
Missall, Tricia A., et al. “Posttranslational, translational, and transcriptional responses to nitric oxide stress in Cryptococcus neoformans: implications for virulence.Eukaryot Cell, vol. 5, no. 3, Mar. 2006, pp. 518–29. Pubmed, doi:10.1128/EC.5.3.518-529.2006.
Missall TA, Pusateri ME, Donlin MJ, Chambers KT, Corbett JA, Lodge JK. Posttranslational, translational, and transcriptional responses to nitric oxide stress in Cryptococcus neoformans: implications for virulence. Eukaryot Cell. 2006 Mar;5(3):518–529.

Published In

Eukaryot Cell

DOI

ISSN

1535-9778

Publication Date

March 2006

Volume

5

Issue

3

Start / End Page

518 / 529

Location

United States

Related Subject Headings

  • Virulence
  • Transcription, Genetic
  • Transaldolase
  • Proteomics
  • Protein Processing, Post-Translational
  • Peroxidases
  • Oxidative Stress
  • Nitric Oxide
  • Microbiology
  • Mice, Inbred CBA