Skip to main content
Journal cover image

Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans.

Publication ,  Journal Article
Gerik, KJ; Donlin, MJ; Soto, CE; Banks, AM; Banks, IR; Maligie, MA; Selitrennikoff, CP; Lodge, JK
Published in: Mol Microbiol
October 2005

Cell wall biogenesis and integrity are crucial for fungal growth, pathogenesis and survival, and are attractive targets for antifungal therapy. In this study, we identify, delete and analyse mutant strains for 10 genes involved in the PKC1 signal transduction pathway and its regulation in Cryptococcus neoformans. The kinases Bck1 and Mkk2 are critical for maintaining integrity, and deletion of each of these causes severe phenotypes different from each other. In stark contrast to results seen in Saccharomyces cerevisiae, a deletion in LRG1 has severe repercussions for the cell, and one in ROM2 has little effect. Also surprisingly, the phosphatase Ppg1 is crucial for cell integrity. These data indicate that the mechanisms of maintaining cell integrity differ between the two fungi. Deletions in SSD1 and PUF4, potential alternative regulators of cell integrity, also exhibit phenotypes. This is the first comprehensive analysis examining genes involved the maintenance of cell integrity in C. neoformans and sets the foundation for future biochemical and virulence studies.

Duke Scholars

Published In

Mol Microbiol

DOI

ISSN

0950-382X

Publication Date

October 2005

Volume

58

Issue

2

Start / End Page

393 / 408

Location

England

Related Subject Headings

  • Trypan Blue
  • Temperature
  • Signal Transduction
  • Saccharomyces cerevisiae Proteins
  • Protein Kinase C
  • Peptides, Cyclic
  • Microbiology
  • Melanins
  • Lipopeptides
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gerik, K. J., Donlin, M. J., Soto, C. E., Banks, A. M., Banks, I. R., Maligie, M. A., … Lodge, J. K. (2005). Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans. Mol Microbiol, 58(2), 393–408. https://doi.org/10.1111/j.1365-2958.2005.04843.x
Gerik, Kimberly J., Maureen J. Donlin, Carlos E. Soto, Annette M. Banks, Isaac R. Banks, Marybeth A. Maligie, Claude P. Selitrennikoff, and Jennifer K. Lodge. “Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans.Mol Microbiol 58, no. 2 (October 2005): 393–408. https://doi.org/10.1111/j.1365-2958.2005.04843.x.
Gerik KJ, Donlin MJ, Soto CE, Banks AM, Banks IR, Maligie MA, et al. Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans. Mol Microbiol. 2005 Oct;58(2):393–408.
Gerik, Kimberly J., et al. “Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans.Mol Microbiol, vol. 58, no. 2, Oct. 2005, pp. 393–408. Pubmed, doi:10.1111/j.1365-2958.2005.04843.x.
Gerik KJ, Donlin MJ, Soto CE, Banks AM, Banks IR, Maligie MA, Selitrennikoff CP, Lodge JK. Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans. Mol Microbiol. 2005 Oct;58(2):393–408.
Journal cover image

Published In

Mol Microbiol

DOI

ISSN

0950-382X

Publication Date

October 2005

Volume

58

Issue

2

Start / End Page

393 / 408

Location

England

Related Subject Headings

  • Trypan Blue
  • Temperature
  • Signal Transduction
  • Saccharomyces cerevisiae Proteins
  • Protein Kinase C
  • Peptides, Cyclic
  • Microbiology
  • Melanins
  • Lipopeptides
  • Humans