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Bitter Taste Receptors and Chronic Otitis Media.

Publication ,  Journal Article
Kaufman, AC; Colquitt, L; Ruckenstein, MJ; Bigelow, DC; Eliades, SJ; Xiong, G; Lin, C; Reed, DR; Cohen, NA
Published in: Otolaryngol Head Neck Surg
August 2021

OBJECTIVE: To evaluate the presence of bitter taste receptors (T2Rs) in the middle ear and to examine their relationship with chronic ear infections. STUDY DESIGN: Cross-sectional study. SETTING: Tertiary care hospital. METHODS: This study enrolled 84 patients being evaluated for otologic surgery: 40 for chronic otitis media (COM) and 44 for other surgical procedures (controls). We collected a small piece of mucosa from 14 patients for mRNA analysis and from 23 patients for immunohistochemistry. A total of 55 patients underwent a double-blind taste test to gauge sensitivity to phenylthiocarbamide, denatonium, quinine, sucrose, and sodium chloride; 47 patients gave a salivary sample for single-nucleotide polymorphism analysis of rs1376251 (TAS2R50) and rs1726866 (TAS2R38). RESULTS: Bitter taste receptors were found in all samples, but the repertoire varied among patients. T2R50 was the most consistently identified receptor by mRNA analysis. Its rs1376251 allele was related to susceptibility to COM but not the expression pattern of T2R50. Ratings of bitterness intensity of phenylthiocarbamide, a ligand for T2R38, differed significantly between the COM and control groups. CONCLUSION: T2Rs were found within the middle ear of every patient sampled; the rs1376251 allele of TAS2R50 appears to be related to chronic ear infections. These receptors are an intriguing target for future research and possible drug targeting.

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Published In

Otolaryngol Head Neck Surg

DOI

EISSN

1097-6817

Publication Date

August 2021

Volume

165

Issue

2

Start / End Page

290 / 299

Location

England

Related Subject Headings

  • Young Adult
  • Taste Perception
  • Taste Disorders
  • Receptors, G-Protein-Coupled
  • RNA, Messenger
  • Polymorphism, Single Nucleotide
  • Otorhinolaryngology
  • Otitis Media
  • Middle Aged
  • Male
 

Citation

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Kaufman, A. C., Colquitt, L., Ruckenstein, M. J., Bigelow, D. C., Eliades, S. J., Xiong, G., … Cohen, N. A. (2021). Bitter Taste Receptors and Chronic Otitis Media. Otolaryngol Head Neck Surg, 165(2), 290–299. https://doi.org/10.1177/0194599820984788
Kaufman, Adam C., Lauren Colquitt, Michael J. Ruckenstein, Douglas C. Bigelow, Steven J. Eliades, Guoxiang Xiong, Cailu Lin, Danielle R. Reed, and Noam A. Cohen. “Bitter Taste Receptors and Chronic Otitis Media.Otolaryngol Head Neck Surg 165, no. 2 (August 2021): 290–99. https://doi.org/10.1177/0194599820984788.
Kaufman AC, Colquitt L, Ruckenstein MJ, Bigelow DC, Eliades SJ, Xiong G, et al. Bitter Taste Receptors and Chronic Otitis Media. Otolaryngol Head Neck Surg. 2021 Aug;165(2):290–9.
Kaufman, Adam C., et al. “Bitter Taste Receptors and Chronic Otitis Media.Otolaryngol Head Neck Surg, vol. 165, no. 2, Aug. 2021, pp. 290–99. Pubmed, doi:10.1177/0194599820984788.
Kaufman AC, Colquitt L, Ruckenstein MJ, Bigelow DC, Eliades SJ, Xiong G, Lin C, Reed DR, Cohen NA. Bitter Taste Receptors and Chronic Otitis Media. Otolaryngol Head Neck Surg. 2021 Aug;165(2):290–299.
Journal cover image

Published In

Otolaryngol Head Neck Surg

DOI

EISSN

1097-6817

Publication Date

August 2021

Volume

165

Issue

2

Start / End Page

290 / 299

Location

England

Related Subject Headings

  • Young Adult
  • Taste Perception
  • Taste Disorders
  • Receptors, G-Protein-Coupled
  • RNA, Messenger
  • Polymorphism, Single Nucleotide
  • Otorhinolaryngology
  • Otitis Media
  • Middle Aged
  • Male