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Self-Assembled Single-Stranded DNA Nano-Networks in Solution and at Surfaces.

Publication ,  Journal Article
Simon, M; Prause, A; Zauscher, S; Gradzielski, M
Published in: Biomacromolecules
March 2022

We studied the directed self-assembly of two types of complementary single-stranded DNA (ssDNA) strands [i.e., poly(dA) and poly(dT)] into more complex, organized, and percolating networks in dilute solutions and at surfaces. Understanding ssDNA self-assembly into 2D networks on surfaces is important for the use of such networks in the fabrication of well-defined nanotechnological devices, as, for instance, required in nanoelectronics or for biosensing. To control the formation of 2D networks on surfaces, it is important to know whether DNA assemblies are formed already in dilute solutions or only during the drying/immobilization process at the surface, where the concentration automatically increases. Fluorescence cross-correlation spectroscopy clearly shows the presence of larger DNA complexes in mixed poly(dA) and poly(dT) solutions already at very low DNA concentrations (<1 nM), that is, well below the overlap concentration. Here, we describe for the first time such supramolecular complexes in solution and how their structure depends on the ssDNA length and concentration and ionic strength. Hence, future attempts to control such networks should also focus on network precursors in solution and not only on their immobilization on surfaces.

Duke Scholars

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

March 2022

Volume

23

Issue

3

Start / End Page

1242 / 1250

Related Subject Headings

  • Spectrometry, Fluorescence
  • Polymers
  • Poly A
  • Osmolar Concentration
  • DNA, Single-Stranded
  • DNA
  • 40 Engineering
  • 34 Chemical sciences
  • 31 Biological sciences
  • 09 Engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Simon, M., Prause, A., Zauscher, S., & Gradzielski, M. (2022). Self-Assembled Single-Stranded DNA Nano-Networks in Solution and at Surfaces. Biomacromolecules, 23(3), 1242–1250. https://doi.org/10.1021/acs.biomac.1c01493
Simon, Miriam, Albert Prause, Stefan Zauscher, and Michael Gradzielski. “Self-Assembled Single-Stranded DNA Nano-Networks in Solution and at Surfaces.Biomacromolecules 23, no. 3 (March 2022): 1242–50. https://doi.org/10.1021/acs.biomac.1c01493.
Simon M, Prause A, Zauscher S, Gradzielski M. Self-Assembled Single-Stranded DNA Nano-Networks in Solution and at Surfaces. Biomacromolecules. 2022 Mar;23(3):1242–50.
Simon, Miriam, et al. “Self-Assembled Single-Stranded DNA Nano-Networks in Solution and at Surfaces.Biomacromolecules, vol. 23, no. 3, Mar. 2022, pp. 1242–50. Epmc, doi:10.1021/acs.biomac.1c01493.
Simon M, Prause A, Zauscher S, Gradzielski M. Self-Assembled Single-Stranded DNA Nano-Networks in Solution and at Surfaces. Biomacromolecules. 2022 Mar;23(3):1242–1250.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

March 2022

Volume

23

Issue

3

Start / End Page

1242 / 1250

Related Subject Headings

  • Spectrometry, Fluorescence
  • Polymers
  • Poly A
  • Osmolar Concentration
  • DNA, Single-Stranded
  • DNA
  • 40 Engineering
  • 34 Chemical sciences
  • 31 Biological sciences
  • 09 Engineering