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A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly.

Publication ,  Journal Article
Andrade, TAD; Evangelista, AF; Campos, AHF; Poles, WA; Borges, NM; Camillo, CMC; Soares, FA; Vassallo, J; Paes, RP; Zerbini, MC; Alves, AC ...
Published in: Oncotarget
December 15, 2014

Currently, there is no characteristic microRNA (miRNA) expression pattern in Epstein-Barr virus+ diffuse large B-cell lymphoma of the elderly (EBV+DLBCLe). This study aims to characterize a signature profile and identify miRNAs that can be used as biomarkers and alternative therapeutic targets for EBV+DLBCLe. Seventy-one DLBCL patients aged 50 years and older were included and four EBV+ and four EBV- samples were analyzed in two miRNA array platforms (pilot study). A larger multicenter cohort (29 EBV+DLBCLe and 65 EBV-DLBCL patients) was used to validate the results by real-time polymerase chain reaction. In the pilot study, 9% of DLBCL were EBV+DLBCLe by in situ hybridization. In multicenter study, EBV+DLBCLe group showed a predominance of non-germinal center B-cell origin. Overall survival duration of EBV+DLBCLe was significantly inferior to that of EBV-DLBCL patients. We found 10 deregulated miRNAs in the two groups, but only seven were statistically different. We confirmed overexpression of hsa-miR-126, hsa-miR-146a, hsa-miR-146b, hsa-miR-150, and hsa-miR-222 and underexpression of hsa-miR-151 in EBV+DLBCLe cases compared to EBV-DLBCL cases. Hsa-miR-146b and hsa-miR-222 showed high specificity for identifying EBV+DLBCLe. The present study proposed a miRNA signature for EBV+DLBCLe and our findings suggest that hsa-miR-146b and hsa-miR-222 could be biomarkers and therapeutic targets.

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Published In

Oncotarget

DOI

EISSN

1949-2553

Publication Date

December 15, 2014

Volume

5

Issue

23

Start / End Page

11813 / 11826

Location

United States

Related Subject Headings

  • Transcriptome
  • Tissue Array Analysis
  • Retrospective Studies
  • Real-Time Polymerase Chain Reaction
  • ROC Curve
  • Pilot Projects
  • Middle Aged
  • MicroRNAs
  • Male
  • Lymphoma, Large B-Cell, Diffuse
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Andrade, T. A. D., Evangelista, A. F., Campos, A. H. F., Poles, W. A., Borges, N. M., Camillo, C. M. C., … Colleoni, G. W. B. (2014). A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly. Oncotarget, 5(23), 11813–11826. https://doi.org/10.18632/oncotarget.2952
Andrade, Tathiana Azevedo de, Adriane Feijo Evangelista, Antonio Hugo Froes Campos, Wagner Augusto Poles, Natalia Morais Borges, Claudia Malheiros Coutinho Camillo, Fernando Augusto Soares, et al. “A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly.Oncotarget 5, no. 23 (December 15, 2014): 11813–26. https://doi.org/10.18632/oncotarget.2952.
Andrade TAD, Evangelista AF, Campos AHF, Poles WA, Borges NM, Camillo CMC, et al. A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly. Oncotarget. 2014 Dec 15;5(23):11813–26.
Andrade, Tathiana Azevedo de, et al. “A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly.Oncotarget, vol. 5, no. 23, Dec. 2014, pp. 11813–26. Pubmed, doi:10.18632/oncotarget.2952.
Andrade TAD, Evangelista AF, Campos AHF, Poles WA, Borges NM, Camillo CMC, Soares FA, Vassallo J, Paes RP, Zerbini MC, Scapulatempo C, Alves AC, Young KH, Colleoni GWB. A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly. Oncotarget. 2014 Dec 15;5(23):11813–11826.

Published In

Oncotarget

DOI

EISSN

1949-2553

Publication Date

December 15, 2014

Volume

5

Issue

23

Start / End Page

11813 / 11826

Location

United States

Related Subject Headings

  • Transcriptome
  • Tissue Array Analysis
  • Retrospective Studies
  • Real-Time Polymerase Chain Reaction
  • ROC Curve
  • Pilot Projects
  • Middle Aged
  • MicroRNAs
  • Male
  • Lymphoma, Large B-Cell, Diffuse