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A reference tissue atlas for the human kidney.

Publication ,  Journal Article
Hansen, J; Sealfon, R; Menon, R; Eadon, MT; Lake, BB; Steck, B; Anjani, K; Parikh, S; Sigdel, TK; Zhang, G; Velickovic, D; Barwinska, D ...
Published in: Sci Adv
June 10, 2022

Kidney Precision Medicine Project (KPMP) is building a spatially specified human kidney tissue atlas in health and disease with single-cell resolution. Here, we describe the construction of an integrated reference map of cells, pathways, and genes using unaffected regions of nephrectomy tissues and undiseased human biopsies from 56 adult subjects. We use single-cell/nucleus transcriptomics, subsegmental laser microdissection transcriptomics and proteomics, near-single-cell proteomics, 3D and CODEX imaging, and spatial metabolomics to hierarchically identify genes, pathways, and cells. Integrated data from these different technologies coherently identify cell types/subtypes within different nephron segments and the interstitium. These profiles describe cell-level functional organization of the kidney following its physiological functions and link cell subtypes to genes, proteins, metabolites, and pathways. They further show that messenger RNA levels along the nephron are congruent with the subsegmental physiological activity. This reference atlas provides a framework for the classification of kidney disease when multiple molecular mechanisms underlie convergent clinical phenotypes.

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Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

June 10, 2022

Volume

8

Issue

23

Start / End Page

eabn4965

Location

United States

Related Subject Headings

  • Transcriptome
  • Proteomics
  • Metabolomics
  • Kidney Diseases
  • Kidney
  • Humans
 

Citation

APA
Chicago
ICMJE
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Hansen, J., Sealfon, R., Menon, R., Eadon, M. T., Lake, B. B., Steck, B., … Kidney Precision Medicine Project, . (2022). A reference tissue atlas for the human kidney. Sci Adv, 8(23), eabn4965. https://doi.org/10.1126/sciadv.abn4965
Hansen, Jens, Rachel Sealfon, Rajasree Menon, Michael T. Eadon, Blue B. Lake, Becky Steck, Kavya Anjani, et al. “A reference tissue atlas for the human kidney.Sci Adv 8, no. 23 (June 10, 2022): eabn4965. https://doi.org/10.1126/sciadv.abn4965.
Hansen J, Sealfon R, Menon R, Eadon MT, Lake BB, Steck B, et al. A reference tissue atlas for the human kidney. Sci Adv. 2022 Jun 10;8(23):eabn4965.
Hansen, Jens, et al. “A reference tissue atlas for the human kidney.Sci Adv, vol. 8, no. 23, June 2022, p. eabn4965. Pubmed, doi:10.1126/sciadv.abn4965.
Hansen J, Sealfon R, Menon R, Eadon MT, Lake BB, Steck B, Anjani K, Parikh S, Sigdel TK, Zhang G, Velickovic D, Barwinska D, Alexandrov T, Dobi D, Rashmi P, Otto EA, Rivera M, Rose MP, Anderton CR, Shapiro JP, Pamreddy A, Winfree S, Xiong Y, He Y, de Boer IH, Hodgin JB, Barisoni L, Naik AS, Sharma K, Sarwal MM, Zhang K, Himmelfarb J, Rovin B, El-Achkar TM, Laszik Z, He JC, Dagher PC, Valerius MT, Jain S, Satlin LM, Troyanskaya OG, Kretzler M, Iyengar R, Azeloglu EU, Kidney Precision Medicine Project. A reference tissue atlas for the human kidney. Sci Adv. 2022 Jun 10;8(23):eabn4965.

Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

June 10, 2022

Volume

8

Issue

23

Start / End Page

eabn4965

Location

United States

Related Subject Headings

  • Transcriptome
  • Proteomics
  • Metabolomics
  • Kidney Diseases
  • Kidney
  • Humans