Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis.
The formation of haploid gametes from diploid germ cells requires the regulated two-step release of sister chromatid cohesion (SCC) during the meiotic divisions. Here, we show that phosphorylation of cohesin subunit REC-8 by Aurora B promotes SCC release at anaphase I onset in C. elegans oocytes. Aurora B loading to chromatin displaying Haspin-mediated H3 T3 phosphorylation induces spatially restricted REC-8 phosphorylation, preventing full SCC release during anaphase I. H3 T3 phosphorylation is locally antagonized by protein phosphatase 1, which is recruited to chromosomes by HTP-1/2 and LAB-1. Mutating the N terminus of HTP-1 causes ectopic H3 T3 phosphorylation, triggering precocious SCC release without impairing earlier HTP-1 roles in homolog pairing and recombination. CDK-1 exerts temporal regulation of Aurora B recruitment, coupling REC-8 phosphorylation to oocyte maturation. Our findings elucidate a complex regulatory network that uses chromosome axis components, H3 T3 phosphorylation, and cell cycle regulators to ensure accurate chromosome segregation during oogenesis.
Duke Scholars
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Related Subject Headings
- Protein Serine-Threonine Kinases
- Protein Phosphatase 1
- Phosphorylation
- Oogenesis
- Oocytes
- Histones
- Hermaphroditic Organisms
- Gene Expression Regulation
- Chromosome Segregation
- Chromosomal Proteins, Non-Histone
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Protein Serine-Threonine Kinases
- Protein Phosphatase 1
- Phosphorylation
- Oogenesis
- Oocytes
- Histones
- Hermaphroditic Organisms
- Gene Expression Regulation
- Chromosome Segregation
- Chromosomal Proteins, Non-Histone