Scholars@Duke publication: Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation.

Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation.

Publication , Journal Article

Matsumoto, J; Kawana, K; Nagamatsu, T; Schust, DJ; Fujii, T; Sato, H; Hyodo, H; Yasugi, T; Kozuma, S; Taketani, Y

Published in: Biochem Biophys Res Commun

June 27, 2008

CD1d is a specific ligand for the invariant Valpha24Vbeta11-natural killer T (iNKT) cells that play an important role in placental development during early human pregnancy. The localization and regulation of placental CD1d expression remain unclear. Immunohistochemistry of human early gestational placentas revealed CD1d was present in villous and extravillous trophoblast (EVT) but not in syncytiotrophoblast or decidual cells. CD1d immunoreactivity in EVT cells decreased with EVT differentiation. Flow cytometry of primary cultured human trophoblast cells confirmed cell-surface expression of CD1d decreased with time in culture. These changes in CD1d expression occur at the level of transcription. TGF-beta1 secreted from the cultured EVT cells accumulated with time in culture and directly suppressed CD1d expression, as evidenced by monoclonal antibody neutralization of TGF-beta1 effects. Thus, trophoblast differentiation is characterized by TGF-beta1-mediated decreases in trophoblast cell CD1d expression. This effect may support appropriate activation of decidual iNKT cells at the maternal-fetal interface.

Duke Scholars

Author Danny J Schust Obstetrics and Gynecology, Reproductive Endocrinology & Infe ...

Published In

Biochem Biophys Res Commun

DOI

10.1016/j.bbrc.2008.04.051

EISSN

1090-2104

Publication Date

June 27, 2008

Volume

371

Issue

Start / End Page

236 / 241

Location

United States

Related Subject Headings

Trophoblasts
Transforming Growth Factor beta1
Transcription, Genetic
Pregnancy
Lymphocyte Activation
Killer Cells, Natural
Humans
Gene Expression Regulation
Female
Down-Regulation

Citation

APA

Chicago

ICMJE

MLA

NLM

Matsumoto, J., Kawana, K., Nagamatsu, T., Schust, D. J., Fujii, T., Sato, H., … Taketani, Y. (2008). Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation. Biochem Biophys Res Commun, 371(2), 236–241. https://doi.org/10.1016/j.bbrc.2008.04.051

Matsumoto, Junko, Kei Kawana, Takeshi Nagamatsu, Danny J. Schust, Tomoyuki Fujii, Hidetaka Sato, Hironobu Hyodo, Toshiharu Yasugi, Shiro Kozuma, and Yuji Taketani. “Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation.” Biochem Biophys Res Commun 371, no. 2 (June 27, 2008): 236–41. https://doi.org/10.1016/j.bbrc.2008.04.051.

Matsumoto J, Kawana K, Nagamatsu T, Schust DJ, Fujii T, Sato H, et al. Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation. Biochem Biophys Res Commun. 2008 Jun 27;371(2):236–41.

Matsumoto, Junko, et al. “Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation.” Biochem Biophys Res Commun, vol. 371, no. 2, June 2008, pp. 236–41. Pubmed, doi:10.1016/j.bbrc.2008.04.051.

Matsumoto J, Kawana K, Nagamatsu T, Schust DJ, Fujii T, Sato H, Hyodo H, Yasugi T, Kozuma S, Taketani Y. Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation. Biochem Biophys Res Commun. 2008 Jun 27;371(2):236–241.