Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis.
Publication
, Journal Article
Seo, YS; Kim, EY; Kim, JH; Kim, WT
Published in: FEBS letters
July 2009
In Arabidopsis, there are at least seven class I acylhydrolase members, which have a putative N-terminal chloroplast-targeting signal. Here, we show that all seven class I proteins are localized to the chloroplasts and hydrolyze phosphatidylcholine at the sn-1 position. However, based on their activities toward various lipids, Arabidopsis class I enzymes could be further divided into three sub-groups by substrate specificity, one with phospholipase-specific activity, another with phospholipase and galactolipase activities, and the other with broad lipolytic activity toward phosphatidylcholine, galactolipids, and triacylglycerol. These results suggest that the three sub-groups of class I acylhydrolases have specific roles in chloroplasts.
Duke Scholars
Published In
FEBS letters
DOI
EISSN
1873-3468
ISSN
0014-5793
Publication Date
July 2009
Volume
583
Issue
13
Start / End Page
2301 / 2307
Related Subject Headings
- Substrate Specificity
- Phospholipases A1
- Hydrolases
- Chloroplasts
- Biochemistry & Molecular Biology
- Arabidopsis Proteins
- Arabidopsis
- 3101 Biochemistry and cell biology
- 0603 Evolutionary Biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Seo, Y. S., Kim, E. Y., Kim, J. H., & Kim, W. T. (2009). Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis. FEBS Letters, 583(13), 2301–2307. https://doi.org/10.1016/j.febslet.2009.06.021
Seo, Young Sam, Eun Yu Kim, Jeong Hoe Kim, and Woo Taek Kim. “Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis.” FEBS Letters 583, no. 13 (July 2009): 2301–7. https://doi.org/10.1016/j.febslet.2009.06.021.
Seo YS, Kim EY, Kim JH, Kim WT. Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis. FEBS letters. 2009 Jul;583(13):2301–7.
Seo, Young Sam, et al. “Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis.” FEBS Letters, vol. 583, no. 13, July 2009, pp. 2301–07. Epmc, doi:10.1016/j.febslet.2009.06.021.
Seo YS, Kim EY, Kim JH, Kim WT. Enzymatic characterization of class I DAD1-like acylhydrolase members targeted to chloroplast in Arabidopsis. FEBS letters. 2009 Jul;583(13):2301–2307.
Published In
FEBS letters
DOI
EISSN
1873-3468
ISSN
0014-5793
Publication Date
July 2009
Volume
583
Issue
13
Start / End Page
2301 / 2307
Related Subject Headings
- Substrate Specificity
- Phospholipases A1
- Hydrolases
- Chloroplasts
- Biochemistry & Molecular Biology
- Arabidopsis Proteins
- Arabidopsis
- 3101 Biochemistry and cell biology
- 0603 Evolutionary Biology
- 0601 Biochemistry and Cell Biology