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Characterization of three Arabidopsis homologs of human RING membrane anchor E3 ubiquitin ligase.

Publication ,  Journal Article
Son, O; Cho, SK; Kim, EY; Kim, WT
Published in: Plant cell reports
April 2009

Ubiquitination affects diverse physiological processes in eukaryotic cells. AtRMA1 was previously identified as an Arabidopsis homolog of human RING membrane-anchor E3 ubiquitin (Ub) ligase. Here, we identified two additional AtRMA homologs, AtRMA2 and AtRMA3. The predicted AtRMA proteins contain a RING motif and a trans-membrane domain in their N-terminal and extreme C-terminal regions, respectively. Bacterially expressed AtRMAs exhibited E3 ligase activity in vitro, which was abrogated by mutation of the conserved cysteine residue in their RING domains. In vivo targeting experiments using an Arabidopsis protoplast-transfection system showed that all three AtRMAs are localized to the ER. Although RT-PCR analysis indicated that AtRMA mRNAs were expressed constitutively in all tissues examined, their promoter activities were differentially detected in a tissue-specific fashion in AtRMA-promoter::GUS transgenic Arabidopsis plants. The AtRMA1 and AtRMA3 genes are predominantly expressed in major tissues, such as cotyledons, leaves, shoot-root junction, roots, and anthers, while AtRMA2 expression is restricted to the root tips and leaf hydathodes. We suggest that a ubiquitnation pathway involving these AtRMA E3 Ub ligases may play a role in the growth and development of Arabidopsis.

Duke Scholars

Published In

Plant cell reports

DOI

EISSN

1432-203X

ISSN

0721-7714

Publication Date

April 2009

Volume

28

Issue

4

Start / End Page

561 / 569

Related Subject Headings

  • Ubiquitination
  • Ubiquitin-Protein Ligases
  • Sequence Analysis, DNA
  • RNA, Plant
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Plant Biology & Botany
  • Mutation
  • Molecular Sequence Data
  • Membrane Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Son, O., Cho, S. K., Kim, E. Y., & Kim, W. T. (2009). Characterization of three Arabidopsis homologs of human RING membrane anchor E3 ubiquitin ligase. Plant Cell Reports, 28(4), 561–569. https://doi.org/10.1007/s00299-009-0680-8
Son, Ora, Seok Keun Cho, Eun Yu Kim, and Woo Taek Kim. “Characterization of three Arabidopsis homologs of human RING membrane anchor E3 ubiquitin ligase.Plant Cell Reports 28, no. 4 (April 2009): 561–69. https://doi.org/10.1007/s00299-009-0680-8.
Son O, Cho SK, Kim EY, Kim WT. Characterization of three Arabidopsis homologs of human RING membrane anchor E3 ubiquitin ligase. Plant cell reports. 2009 Apr;28(4):561–9.
Son, Ora, et al. “Characterization of three Arabidopsis homologs of human RING membrane anchor E3 ubiquitin ligase.Plant Cell Reports, vol. 28, no. 4, Apr. 2009, pp. 561–69. Epmc, doi:10.1007/s00299-009-0680-8.
Son O, Cho SK, Kim EY, Kim WT. Characterization of three Arabidopsis homologs of human RING membrane anchor E3 ubiquitin ligase. Plant cell reports. 2009 Apr;28(4):561–569.
Journal cover image

Published In

Plant cell reports

DOI

EISSN

1432-203X

ISSN

0721-7714

Publication Date

April 2009

Volume

28

Issue

4

Start / End Page

561 / 569

Related Subject Headings

  • Ubiquitination
  • Ubiquitin-Protein Ligases
  • Sequence Analysis, DNA
  • RNA, Plant
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Plant Biology & Botany
  • Mutation
  • Molecular Sequence Data
  • Membrane Proteins