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Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog.

Publication ,  Journal Article
Seo, YS; Kim, EY; Mang, HG; Kim, WT
Published in: The Plant journal : for cell and molecular biology
March 2008

Phospholipid signaling has been recently implicated in diverse cellular processes in higher plants. We identified a cDNA encoding the phospholipase A1 homolog (CaPLA1) from 5-day-old early roots of hot pepper. The deduced amino acid sequence showed that the lipase-specific catalytic triad is well conserved in CaPLA1. In vitro lipase assays and site-directed mutagenesis revealed that CaPLA1 possesses PLA1 activity, which catalyzes the hydrolysis of phospholipids at the sn-1 position. CaPLA1 was selectively expressed in young roots, at days 4-5 after germination, and rapidly declined thereafter, suggesting that the expression of CaPLA1 is subject to control by a development-specific mechanism in roots. Because transgenic work was extremely difficult in hot peppers, in this study we overexpressed CaPLA1 in Arabidopsis so as to provide cellular information on the function of this gene. CaPLA1 overexpressors had significantly longer roots, leaves and petioles, and grew more rapidly than the wild-type plants, leading to an early bolting phenotype with prolonged inflorescence. Microscopic analysis showed that the vegetative tissues of 35S:CaPLA1 plants contained an increased number of small-sized cells, which resulted in highly populated cell layers. In addition, mRNAs for cell cycle-controlled proteins and fatty acid catabolizing enzymes were coordinately upregulated in CaPLA1-overexpressing plants. These results suggest that CaPLA1 is functionally relevant in heterologous Arabidopsis cells, and hence might participate in a subset of positive control mechanisms of cell and tissue growth in transgenic lines. We discuss possible biochemical and cellular functions of CaPLA1 in relation to the phospholipid signaling pathway in hot pepper and transgenic Arabidopsis plants.

Duke Scholars

Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

March 2008

Volume

53

Issue

6

Start / End Page

895 / 908

Related Subject Headings

  • Up-Regulation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Plants, Genetically Modified
  • Plant Roots
  • Plant Leaves
  • Plant Biology & Botany
  • Phospholipases A1
  • Gene Expression Regulation, Plant
  • Cell Cycle
  • Capsicum
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Seo, Y. S., Kim, E. Y., Mang, H. G., & Kim, W. T. (2008). Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog. The Plant Journal : For Cell and Molecular Biology, 53(6), 895–908. https://doi.org/10.1111/j.1365-313x.2007.03380.x
Seo, Young Sam, Eun Yu Kim, Hyung Gon Mang, and Woo Taek Kim. “Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog.The Plant Journal : For Cell and Molecular Biology 53, no. 6 (March 2008): 895–908. https://doi.org/10.1111/j.1365-313x.2007.03380.x.
Seo YS, Kim EY, Mang HG, Kim WT. Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog. The Plant journal : for cell and molecular biology. 2008 Mar;53(6):895–908.
Seo, Young Sam, et al. “Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog.The Plant Journal : For Cell and Molecular Biology, vol. 53, no. 6, Mar. 2008, pp. 895–908. Epmc, doi:10.1111/j.1365-313x.2007.03380.x.
Seo YS, Kim EY, Mang HG, Kim WT. Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog. The Plant journal : for cell and molecular biology. 2008 Mar;53(6):895–908.
Journal cover image

Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

March 2008

Volume

53

Issue

6

Start / End Page

895 / 908

Related Subject Headings

  • Up-Regulation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Plants, Genetically Modified
  • Plant Roots
  • Plant Leaves
  • Plant Biology & Botany
  • Phospholipases A1
  • Gene Expression Regulation, Plant
  • Cell Cycle
  • Capsicum