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3D patterned stem cell differentiation using thermo-responsive methylcellulose hydrogel molds.

Publication ,  Journal Article
Lee, W; Park, J
Published in: Sci Rep
July 6, 2016

Tissue-specific patterned stem cell differentiation serves as the basis for the development, remodeling, and regeneration of the multicellular structure of the native tissues. We herein proposed a cytocompatible 3D casting process to recapitulate this patterned stem cell differentiation for reconstructing multicellular tissues in vitro. We first reconstituted the 2D culture conditions for stem cell fate control within 3D hydrogel by incorporating the sets of the diffusible signal molecules delivered through drug-releasing microparticles. Then, utilizing thermo-responsivity of methylcellulose (MC), we developed a cytocompatible casting process to mold these hydrogels into specific 3D configurations, generating the targeted spatial gradients of diffusible signal molecules. The liquid phase of the MC solution was viscous enough to adopt the shapes of 3D impression patterns, while the gelated MC served as a reliable mold for patterning the hydrogel prepolymers. When these patterned hydrogels were integrated together, the stem cells in each hydrogel distinctly differentiated toward individually defined fates, resulting in the formation of the multicellular tissue structure bearing the very structural integrity and characteristics as seen in vascularized bones and osteochondral tissues.

Duke Scholars

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

July 6, 2016

Volume

6

Start / End Page

29408

Location

England

Related Subject Headings

  • Viscosity
  • Thermodynamics
  • Stem Cells
  • Methylcellulose
  • Hydrogels
  • Humans
  • Cells, Cultured
  • Cell Differentiation
  • Cell Culture Techniques
 

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Lee, W., & Park, J. (2016). 3D patterned stem cell differentiation using thermo-responsive methylcellulose hydrogel molds. Sci Rep, 6, 29408. https://doi.org/10.1038/srep29408
Lee, Wonjae, and Jon Park. “3D patterned stem cell differentiation using thermo-responsive methylcellulose hydrogel molds.Sci Rep 6 (July 6, 2016): 29408. https://doi.org/10.1038/srep29408.
Lee, Wonjae, and Jon Park. “3D patterned stem cell differentiation using thermo-responsive methylcellulose hydrogel molds.Sci Rep, vol. 6, July 2016, p. 29408. Pubmed, doi:10.1038/srep29408.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

July 6, 2016

Volume

6

Start / End Page

29408

Location

England

Related Subject Headings

  • Viscosity
  • Thermodynamics
  • Stem Cells
  • Methylcellulose
  • Hydrogels
  • Humans
  • Cells, Cultured
  • Cell Differentiation
  • Cell Culture Techniques