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Characterization of a novel two-component system in Burkholderia cenocepacia.

Publication ,  Journal Article
Merry, CR; Perkins, M; Mu, L; Peterson, BK; Knackstedt, RW; Weingart, CL
Published in: Curr Microbiol
April 2015

Two-component systems are important regulatory systems that allow bacteria to adjust to environmental conditions, and in some bacteria are used in pathogenesis. We identified a novel two-component system in Burkholderia cenocepacia, an opportunistic pathogen that causes pneumonia in cystic fibrosis (CF) patients. The putative operon encodes BceS, a sensor kinase, and BceR, a response regulator. Our studies indicated that the bceR mutant showed a statistically significant decrease in protease, swimming motility, and quorum sensing when compared to the wild-type, but there was no significant difference in phospholipase C activity, swarming, and biofilm formation. In addition, the mutant showed a statistically significant reduction in virulence compared to the wild-type using the alfalfa plant model. Examination of the Burkholderia cepacia complex (a group of organisms that are phenotypically similar, but genotypically distinct) revealed that this system is prevalent in B. ambifaria, B. multivorans, B. vietnamiensis and B. dolosa. Interestingly, all these organisms have been associated with CF patients. The collective results indicate that BceSR influences various activities important in Burkholderia physiology and possibly pathogenesis. This information could be important in the design of novel therapeutics for Burkholderia infections.

Duke Scholars

Published In

Curr Microbiol

DOI

EISSN

1432-0991

Publication Date

April 2015

Volume

70

Issue

4

Start / End Page

556 / 561

Location

United States

Related Subject Headings

  • Virulence
  • Transcription Factors
  • Signal Transduction
  • Protein Kinases
  • Plant Diseases
  • Operon
  • Mutation
  • Microbiology
  • Medicago sativa
  • Histidine Kinase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Merry, C. R., Perkins, M., Mu, L., Peterson, B. K., Knackstedt, R. W., & Weingart, C. L. (2015). Characterization of a novel two-component system in Burkholderia cenocepacia. Curr Microbiol, 70(4), 556–561. https://doi.org/10.1007/s00284-014-0744-z
Merry, Callie R., Michael Perkins, Lin Mu, Bridget K. Peterson, Rebecca W. Knackstedt, and Christine L. Weingart. “Characterization of a novel two-component system in Burkholderia cenocepacia.Curr Microbiol 70, no. 4 (April 2015): 556–61. https://doi.org/10.1007/s00284-014-0744-z.
Merry CR, Perkins M, Mu L, Peterson BK, Knackstedt RW, Weingart CL. Characterization of a novel two-component system in Burkholderia cenocepacia. Curr Microbiol. 2015 Apr;70(4):556–61.
Merry, Callie R., et al. “Characterization of a novel two-component system in Burkholderia cenocepacia.Curr Microbiol, vol. 70, no. 4, Apr. 2015, pp. 556–61. Pubmed, doi:10.1007/s00284-014-0744-z.
Merry CR, Perkins M, Mu L, Peterson BK, Knackstedt RW, Weingart CL. Characterization of a novel two-component system in Burkholderia cenocepacia. Curr Microbiol. 2015 Apr;70(4):556–561.
Journal cover image

Published In

Curr Microbiol

DOI

EISSN

1432-0991

Publication Date

April 2015

Volume

70

Issue

4

Start / End Page

556 / 561

Location

United States

Related Subject Headings

  • Virulence
  • Transcription Factors
  • Signal Transduction
  • Protein Kinases
  • Plant Diseases
  • Operon
  • Mutation
  • Microbiology
  • Medicago sativa
  • Histidine Kinase