An examination of the hexane extract of flue-cured tobacco involving gel permeation chromatography

Techniques have been investigated for separating the hexane extract of flue-cured tobacco into classes based on polarity and molecular size. The method developed, which depends upon counter-current distribution for polarity separation and gel permeation chromatography for molecular size separation, is reproducible, non-destructive, and permits quantitative recovery. Its applicability has been demonstrated by the isolation and identification of several compounds and classes of compounds by following it with conventional chromatographic techniques (column, thin-layer, and gas/liquid chromatography). Among the substances isolated were glycerides, solanesol esters, sterol esters (including cholesterol esters), solanochromene (plastochromenol-8), solanesol, α-tocopherol, β-amyrin, and sterols (including cholesterol). Molecular weight determinations indicated that 70 per cent of the non-basic hexane extract was below 800 in molecular weight. © 1969.

Duke Scholars

Author Chad E. Cook Orthopaedic Surgery, Physical Therapy