The effect of different incubation systems on the in vitro metabolism of norethynodrel
Publication
, Journal Article
Freudenthal, RI; Cook, CE; Rosenfeld, R; Wall, ME
Published in: Journal of Steroid Biochemistry
January 1, 1971
The in vitro metabolism of norethynodrel by male rat liver has been studied under a number of different incubation conditions. Both the postmitochondrial supernatant and the microsomal pellet were used as the enzyme source. Optimum incubation conditions for norethynodrel metabolism have been determined with respect to cofactor requirements. The presence of either sucrose, EDTA, Mg2+ or nicotinamide alone or in combination in the incubation medium did not increase substrate metabolism. Maximum product formation occurred using a simple potassium phosphate buffer and a NADPH regenerating system. © 1971.
Duke Scholars
Published In
Journal of Steroid Biochemistry
DOI
ISSN
0022-4731
Publication Date
January 1, 1971
Volume
2
Issue
1
Start / End Page
77 / 82
Citation
APA
Chicago
ICMJE
MLA
NLM
Freudenthal, R. I., Cook, C. E., Rosenfeld, R., & Wall, M. E. (1971). The effect of different incubation systems on the in vitro metabolism of norethynodrel. Journal of Steroid Biochemistry, 2(1), 77–82. https://doi.org/10.1016/0022-4731(71)90011-2
Freudenthal, R. I., C. E. Cook, R. Rosenfeld, and M. E. Wall. “The effect of different incubation systems on the in vitro metabolism of norethynodrel.” Journal of Steroid Biochemistry 2, no. 1 (January 1, 1971): 77–82. https://doi.org/10.1016/0022-4731(71)90011-2.
Freudenthal RI, Cook CE, Rosenfeld R, Wall ME. The effect of different incubation systems on the in vitro metabolism of norethynodrel. Journal of Steroid Biochemistry. 1971 Jan 1;2(1):77–82.
Freudenthal, R. I., et al. “The effect of different incubation systems on the in vitro metabolism of norethynodrel.” Journal of Steroid Biochemistry, vol. 2, no. 1, Jan. 1971, pp. 77–82. Scopus, doi:10.1016/0022-4731(71)90011-2.
Freudenthal RI, Cook CE, Rosenfeld R, Wall ME. The effect of different incubation systems on the in vitro metabolism of norethynodrel. Journal of Steroid Biochemistry. 1971 Jan 1;2(1):77–82.
Published In
Journal of Steroid Biochemistry
DOI
ISSN
0022-4731
Publication Date
January 1, 1971
Volume
2
Issue
1
Start / End Page
77 / 82