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Highly-automated, high-throughput replication of yeast-based logic circuit design assessments.
Publication
, Journal Article
Goldman, RP; Moseley, R; Roehner, N; Cummins, B; Vrana, JD; Clowers, KJ; Bryce, D; Beal, J; DeHaven, M; Nowak, J; Higa, T; Biggers, V; Lee, P ...
Published in: Synthetic biology (Oxford, England)
January 2022
We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of Saccharomyces cerevisiae by Gander et al. Our experimental campaign used a novel high-throughput experimentation framework developed under Defense Advanced Research Projects Agency's Synergistic Discovery and Design program: a remote robotic lab at Strateos executed a parameterized experimental protocol. Using this protocol and robotic execution, we generated two orders of magnitude more flow cytometry data than the original experiments. We discuss our results, which largely, but not completely, agree with the original report and make some remarks about lessons learned. Graphical Abstract.
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Published In
Synthetic biology (Oxford, England)
DOI
EISSN
2397-7000
ISSN
2397-7000
Publication Date
January 2022
Volume
7
Issue
1
Start / End Page
ysac018
Related Subject Headings
- 4901 Applied mathematics
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
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Goldman, R. P., Moseley, R., Roehner, N., Cummins, B., Vrana, J. D., Clowers, K. J., … Colonna-Romano, J. (2022). Highly-automated, high-throughput replication of yeast-based logic circuit design assessments. Synthetic Biology (Oxford, England), 7(1), ysac018. https://doi.org/10.1093/synbio/ysac018
Goldman, Robert P., Robert Moseley, Nicholas Roehner, Breschine Cummins, Justin D. Vrana, Katie J. Clowers, Daniel Bryce, et al. “Highly-automated, high-throughput replication of yeast-based logic circuit design assessments.” Synthetic Biology (Oxford, England) 7, no. 1 (January 2022): ysac018. https://doi.org/10.1093/synbio/ysac018.
Goldman RP, Moseley R, Roehner N, Cummins B, Vrana JD, Clowers KJ, et al. Highly-automated, high-throughput replication of yeast-based logic circuit design assessments. Synthetic biology (Oxford, England). 2022 Jan;7(1):ysac018.
Goldman, Robert P., et al. “Highly-automated, high-throughput replication of yeast-based logic circuit design assessments.” Synthetic Biology (Oxford, England), vol. 7, no. 1, Jan. 2022, p. ysac018. Epmc, doi:10.1093/synbio/ysac018.
Goldman RP, Moseley R, Roehner N, Cummins B, Vrana JD, Clowers KJ, Bryce D, Beal J, DeHaven M, Nowak J, Higa T, Biggers V, Lee P, Hunt JP, Mosqueda L, Haase SB, Weston M, Zheng G, Deckard A, Gopaulakrishnan S, Stubbs JF, Gaffney NI, Vaughn MW, Maheshri N, Mikhalev E, Bartley B, Markeloff R, Mitchell T, Nguyen T, Sumorok D, Walczak N, Myers C, Zundel Z, Hatch B, Scholz J, Colonna-Romano J. Highly-automated, high-throughput replication of yeast-based logic circuit design assessments. Synthetic biology (Oxford, England). 2022 Jan;7(1):ysac018.
![Journal cover image](https://secure.syndetics.com/index.aspx?isbn=/lc.gif&issn=2397-7000&client=dukeuniv)
Published In
Synthetic biology (Oxford, England)
DOI
EISSN
2397-7000
ISSN
2397-7000
Publication Date
January 2022
Volume
7
Issue
1
Start / End Page
ysac018
Related Subject Headings
- 4901 Applied mathematics
- 3101 Biochemistry and cell biology