Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Regulated expression of heparin-binding EGF-like growth factor (HB-EGF) in the human endometrium: a potential paracrine role during implantation.

Publication ,  Journal Article
Lessey, BA; Gui, Y; Apparao, KBC; Young, SL; Mulholland, J
Published in: Mol Reprod Dev
August 2002

Heparin-binding epidermal growth factor (HB-EGF) is a recently identified member of the EGF growth factor family found to be expressed in the uterus of both mouse and human at the time of implantation. In the present study, we investigated the expression patterns of HB-EGF in normal cycling endometrium and compared its expression with the fertility-associated endometrial epithelial biomarkers alpha(v)beta(3) integrin, leukemia inhibitory factor (LIF) and homeobox gene, HOXA-10. RNase protection assay (RPA) using RNA made from endometrium collected from different phases of the menstrual cycle demonstrated increased HB-EGF expression during the mid-secretory phase, a pattern similar to, but slightly preceding the expression of alpha(v)beta(3) integrin and HOXA-10. In vitro studies demonstrated stimulation of HB-EGF expression by estradiol-17beta (E(2)) and progesterone (P(4)) alone or in combination in stromal cells. Combined treatment with E(2) + P(4) was, however, required to stimulate epithelial HB-EGF expression. In vitro experiments demonstrated the ability of HB-EGF to stimulate epithelial expression of the key endometrial proteins including LIF, HOXA-10, and the beta(3) integrin subunit. Each has previously been demonstrated to be an important epithelial biomarker expressed during the implantation window. In addition, conditioned media from endometrial stromal cells treated with E(2) + P(4) + relaxin mimicked the stimulatory effect of HB-EGF on epithelial expression of the beta(3) integrin subunit. The stimulatory effect of the stromal-conditioned medium was blocked by antibodies that neutralize a known receptor for HB-EGF. These data suggest that uterine receptivity may be regulated in part by the stromal-derived HB-EGF.

Duke Scholars

Published In

Mol Reprod Dev

DOI

ISSN

1040-452X

Publication Date

August 2002

Volume

62

Issue

4

Start / End Page

446 / 455

Location

United States

Related Subject Headings

  • Steroids
  • Paracrine Communication
  • Obstetrics & Reproductive Medicine
  • Menstrual Cycle
  • Intercellular Signaling Peptides and Proteins
  • Humans
  • Heparin-binding EGF-like Growth Factor
  • Gene Expression Regulation
  • Female
  • Epidermal Growth Factor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lessey, B. A., Gui, Y., Apparao, K. B. C., Young, S. L., & Mulholland, J. (2002). Regulated expression of heparin-binding EGF-like growth factor (HB-EGF) in the human endometrium: a potential paracrine role during implantation. Mol Reprod Dev, 62(4), 446–455. https://doi.org/10.1002/mrd.10129
Lessey, Bruce A., Yaoting Gui, K. B. C. Apparao, Steven L. Young, and Joy Mulholland. “Regulated expression of heparin-binding EGF-like growth factor (HB-EGF) in the human endometrium: a potential paracrine role during implantation.Mol Reprod Dev 62, no. 4 (August 2002): 446–55. https://doi.org/10.1002/mrd.10129.
Lessey, Bruce A., et al. “Regulated expression of heparin-binding EGF-like growth factor (HB-EGF) in the human endometrium: a potential paracrine role during implantation.Mol Reprod Dev, vol. 62, no. 4, Aug. 2002, pp. 446–55. Pubmed, doi:10.1002/mrd.10129.
Lessey BA, Gui Y, Apparao KBC, Young SL, Mulholland J. Regulated expression of heparin-binding EGF-like growth factor (HB-EGF) in the human endometrium: a potential paracrine role during implantation. Mol Reprod Dev. 2002 Aug;62(4):446–455.
Journal cover image

Published In

Mol Reprod Dev

DOI

ISSN

1040-452X

Publication Date

August 2002

Volume

62

Issue

4

Start / End Page

446 / 455

Location

United States

Related Subject Headings

  • Steroids
  • Paracrine Communication
  • Obstetrics & Reproductive Medicine
  • Menstrual Cycle
  • Intercellular Signaling Peptides and Proteins
  • Humans
  • Heparin-binding EGF-like Growth Factor
  • Gene Expression Regulation
  • Female
  • Epidermal Growth Factor