Scholars@Duke publication: Long range dynamic effects of point-mutations trap a response regulator in an active conformation.

Long range dynamic effects of point-mutations trap a response regulator in an active conformation.

Publication , Journal Article

Bobay, BG; Thompson, RJ; Hoch, JA; Cavanagh, J

Published in: FEBS Lett

October 8, 2010

Published version (DOI) Open Access Copy (Duke) Link to item

When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This 'conformational trapping' results in functionally hyperactive Spo0F. Consequently, point-mutations are seen to affect functionally critical motions both close to and far from the mutational site.

Duke Scholars

Author Benjamin Bobay Radiology

Published In

FEBS Lett

DOI

10.1016/j.febslet.2010.08.051

EISSN

1873-3468

Publication Date

October 8, 2010

Volume

584

Issue

Start / End Page

4203 / 4207

Location

England

Related Subject Headings

Thermodynamics
Signal Transduction
Second Messenger Systems
Protein Conformation
Principal Component Analysis
Point Mutation
Nuclear Magnetic Resonance, Biomolecular
Mutant Proteins
Mutagenesis, Site-Directed
Models, Molecular

Citation

APA

Chicago

ICMJE

MLA

NLM

Bobay, B. G., Thompson, R. J., Hoch, J. A., & Cavanagh, J. (2010). Long range dynamic effects of point-mutations trap a response regulator in an active conformation. FEBS Lett, 584(19), 4203–4207. https://doi.org/10.1016/j.febslet.2010.08.051

Bobay, Benjamin G., Richele J. Thompson, James A. Hoch, and John Cavanagh. “Long range dynamic effects of point-mutations trap a response regulator in an active conformation.” FEBS Lett 584, no. 19 (October 8, 2010): 4203–7. https://doi.org/10.1016/j.febslet.2010.08.051.

Bobay BG, Thompson RJ, Hoch JA, Cavanagh J. Long range dynamic effects of point-mutations trap a response regulator in an active conformation. FEBS Lett. 2010 Oct 8;584(19):4203–7.

Bobay, Benjamin G., et al. “Long range dynamic effects of point-mutations trap a response regulator in an active conformation.” FEBS Lett, vol. 584, no. 19, Oct. 2010, pp. 4203–07. Pubmed, doi:10.1016/j.febslet.2010.08.051.

Bobay BG, Thompson RJ, Hoch JA, Cavanagh J. Long range dynamic effects of point-mutations trap a response regulator in an active conformation. FEBS Lett. 2010 Oct 8;584(19):4203–4207.