Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition.
Publication
, Journal Article
McLaughlin, PD; Bobay, BG; Regel, EJ; Thompson, RJ; Hoch, JA; Cavanagh, J
Published in: FEBS Lett
April 3, 2007
Several alanine mutations in the response regulator Spo0F induce hypersporulation in Bacillus subtilis. L66A, I90A and H101A mutants are purported to be involved in contacts stabilizing the orientation of the alpha4-helix and hence the beta4-alpha4 kinase recognition loop. Y13A is thought to affect the orientation of the alpha1-helix and consequently phosphatase action. Using comparative NMR chemical shift analyses for these mutants, we have confirmed these suppositions and isolated residues in Spo0F critical in sensor kinases discrimination. In addition, we discuss how buried residues and intra-protein communication networks contribute to precise molecular recognition by ensuring that the correct surface is presented.
Duke Scholars
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
April 3, 2007
Volume
581
Issue
7
Start / End Page
1425 / 1429
Location
England
Related Subject Headings
- Spores, Bacterial
- Protein Structure, Secondary
- Protein Kinases
- Protein Interaction Mapping
- Protein Conformation
- Nuclear Magnetic Resonance, Biomolecular
- Histidine Kinase
- Biochemistry & Molecular Biology
- Bacterial Proteins
- Bacillus subtilis
Citation
APA
Chicago
ICMJE
MLA
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McLaughlin, P. D., Bobay, B. G., Regel, E. J., Thompson, R. J., Hoch, J. A., & Cavanagh, J. (2007). Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition. FEBS Lett, 581(7), 1425–1429. https://doi.org/10.1016/j.febslet.2007.02.061
McLaughlin, Patrick D., Benjamin G. Bobay, Erin J. Regel, Richele J. Thompson, James A. Hoch, and John Cavanagh. “Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition.” FEBS Lett 581, no. 7 (April 3, 2007): 1425–29. https://doi.org/10.1016/j.febslet.2007.02.061.
McLaughlin PD, Bobay BG, Regel EJ, Thompson RJ, Hoch JA, Cavanagh J. Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition. FEBS Lett. 2007 Apr 3;581(7):1425–9.
McLaughlin, Patrick D., et al. “Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition.” FEBS Lett, vol. 581, no. 7, Apr. 2007, pp. 1425–29. Pubmed, doi:10.1016/j.febslet.2007.02.061.
McLaughlin PD, Bobay BG, Regel EJ, Thompson RJ, Hoch JA, Cavanagh J. Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition. FEBS Lett. 2007 Apr 3;581(7):1425–1429.
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
April 3, 2007
Volume
581
Issue
7
Start / End Page
1425 / 1429
Location
England
Related Subject Headings
- Spores, Bacterial
- Protein Structure, Secondary
- Protein Kinases
- Protein Interaction Mapping
- Protein Conformation
- Nuclear Magnetic Resonance, Biomolecular
- Histidine Kinase
- Biochemistry & Molecular Biology
- Bacterial Proteins
- Bacillus subtilis