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Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root.

Publication ,  Journal Article
Nolan, TM; Vukašinović, N; Hsu, C-W; Zhang, J; Vanhoutte, I; Shahan, R; Taylor, IW; Greenstreet, L; Heitz, M; Afanassiev, A; Wang, P; Yin, Y ...
Published in: Science (New York, N.Y.)
March 2023

Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall-related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.

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Published In

Science (New York, N.Y.)

DOI

EISSN

1095-9203

ISSN

0036-8075

Publication Date

March 2023

Volume

379

Issue

6639

Start / End Page

eadf4721

Related Subject Headings

  • Transcription Factors
  • Plant Roots
  • Plant Growth Regulators
  • Homeodomain Proteins
  • General Science & Technology
  • Gene Regulatory Networks
  • Gene Expression Regulation, Plant
  • Cell Division
  • Cell Differentiation
  • Brassinosteroids
 

Citation

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Nolan, T. M., Vukašinović, N., Hsu, C.-W., Zhang, J., Vanhoutte, I., Shahan, R., … Benfey, P. N. (2023). Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root. Science (New York, N.Y.), 379(6639), eadf4721. https://doi.org/10.1126/science.adf4721
Nolan, Trevor M., Nemanja Vukašinović, Che-Wei Hsu, Jingyuan Zhang, Isabelle Vanhoutte, Rachel Shahan, Isaiah W. Taylor, et al. “Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root.Science (New York, N.Y.) 379, no. 6639 (March 2023): eadf4721. https://doi.org/10.1126/science.adf4721.
Nolan TM, Vukašinović N, Hsu C-W, Zhang J, Vanhoutte I, Shahan R, et al. Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root. Science (New York, NY). 2023 Mar;379(6639):eadf4721.
Nolan, Trevor M., et al. “Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root.Science (New York, N.Y.), vol. 379, no. 6639, Mar. 2023, p. eadf4721. Epmc, doi:10.1126/science.adf4721.
Nolan TM, Vukašinović N, Hsu C-W, Zhang J, Vanhoutte I, Shahan R, Taylor IW, Greenstreet L, Heitz M, Afanassiev A, Wang P, Szekely P, Brosnan A, Yin Y, Schiebinger G, Ohler U, Russinova E, Benfey PN. Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root. Science (New York, NY). 2023 Mar;379(6639):eadf4721.
Journal cover image

Published In

Science (New York, N.Y.)

DOI

EISSN

1095-9203

ISSN

0036-8075

Publication Date

March 2023

Volume

379

Issue

6639

Start / End Page

eadf4721

Related Subject Headings

  • Transcription Factors
  • Plant Roots
  • Plant Growth Regulators
  • Homeodomain Proteins
  • General Science & Technology
  • Gene Regulatory Networks
  • Gene Expression Regulation, Plant
  • Cell Division
  • Cell Differentiation
  • Brassinosteroids