Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2.
Publication
, Journal Article
Markwalter, CF; Jang, IK; Burton, RA; Domingo, GJ; Wright, DW
Published in: Analytical biochemistry
October 2017
Predicting antibody pair performance in a sandwich format streamlines development of antibody-based diagnostics and laboratory research tools, such as enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFAs). We have evaluated panels of monoclonal antibodies against the malarial parasite biomarker Plasmodium falciparum histidine rich protein 2 (HRP2), including 9 new monoclonal antibodies, using biolayer interferometry (BLI) and screened antibody pairs in a checkerboard ELISA. This study showed BLI predicts antibody pair ELISA performance for HRP2. Pairs that included capture antibodies with low off-rate constants and detection antibodies with high on-rate constants performed best in an ELISA format.
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Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
October 2017
Volume
534
Start / End Page
10 / 13
Related Subject Headings
- Protozoan Proteins
- Plasmodium falciparum
- Enzyme-Linked Immunosorbent Assay
- Biochemistry & Molecular Biology
- Antigens, Protozoan
- Antigen-Antibody Reactions
- Antibodies, Monoclonal
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Markwalter, C. F., Jang, I. K., Burton, R. A., Domingo, G. J., & Wright, D. W. (2017). Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2. Analytical Biochemistry, 534, 10–13. https://doi.org/10.1016/j.ab.2017.07.010
Markwalter, C. F., I. K. Jang, R. A. Burton, G. J. Domingo, and D. W. Wright. “Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2.” Analytical Biochemistry 534 (October 2017): 10–13. https://doi.org/10.1016/j.ab.2017.07.010.
Markwalter CF, Jang IK, Burton RA, Domingo GJ, Wright DW. Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2. Analytical biochemistry. 2017 Oct;534:10–3.
Markwalter, C. F., et al. “Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2.” Analytical Biochemistry, vol. 534, Oct. 2017, pp. 10–13. Epmc, doi:10.1016/j.ab.2017.07.010.
Markwalter CF, Jang IK, Burton RA, Domingo GJ, Wright DW. Biolayer interferometry predicts ELISA performance of monoclonal antibody pairs for Plasmodium falciparum histidine-rich protein 2. Analytical biochemistry. 2017 Oct;534:10–13.
Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
October 2017
Volume
534
Start / End Page
10 / 13
Related Subject Headings
- Protozoan Proteins
- Plasmodium falciparum
- Enzyme-Linked Immunosorbent Assay
- Biochemistry & Molecular Biology
- Antigens, Protozoan
- Antigen-Antibody Reactions
- Antibodies, Monoclonal
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology