Skip to main content

Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions.

Publication ,  Journal Article
Stabryla, LM; Johnston, KA; Diemler, NA; Cooper, VS; Millstone, JE; Haig, S-J; Gilbertson, LM
Published in: Nature nanotechnology
September 2021

Unlike conventional antimicrobials, the study of bacterial resistance to silver nanoparticles (AgNPs) remains in its infancy and the mechanism(s) through which it evolves are limited and inconclusive. The central question remains whether bacterial resistance is driven by the AgNPs, released Ag(I) ions or a combination of these and other factors. Here, we show a specific resistance in an Escherichia coli K-12 MG1655 strain to subinhibitory concentrations of AgNPs, and not Ag(I) ions, as indicated by a statistically significant greater-than-twofold increase in the minimum inhibitory concentration occurring after eight repeated passages that was maintained after the AgNPs were removed and reintroduced. Whole-population genome sequencing identified a cusS mutation associated with the heritable resistance that possibly increased silver ion efflux. Finally, we rule out the effect of particle aggregation on resistance and suggest that the mechanism of resistance may be enhanced or mediated by flagellum-based motility.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nature nanotechnology

DOI

EISSN

1748-3395

ISSN

1748-3387

Publication Date

September 2021

Volume

16

Issue

9

Start / End Page

996 / 1003

Related Subject Headings

  • Silver
  • Nanoscience & Nanotechnology
  • Microbial Sensitivity Tests
  • Metal Nanoparticles
  • Ions
  • Escherichia coli K12
  • Drug Resistance, Bacterial
  • Cell Movement
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Stabryla, L. M., Johnston, K. A., Diemler, N. A., Cooper, V. S., Millstone, J. E., Haig, S.-J., & Gilbertson, L. M. (2021). Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions. Nature Nanotechnology, 16(9), 996–1003. https://doi.org/10.1038/s41565-021-00929-w
Stabryla, Lisa M., Kathryn A. Johnston, Nathan A. Diemler, Vaughn S. Cooper, Jill E. Millstone, Sarah-Jane Haig, and Leanne M. Gilbertson. “Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions.Nature Nanotechnology 16, no. 9 (September 2021): 996–1003. https://doi.org/10.1038/s41565-021-00929-w.
Stabryla LM, Johnston KA, Diemler NA, Cooper VS, Millstone JE, Haig S-J, et al. Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions. Nature nanotechnology. 2021 Sep;16(9):996–1003.
Stabryla, Lisa M., et al. “Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions.Nature Nanotechnology, vol. 16, no. 9, Sept. 2021, pp. 996–1003. Epmc, doi:10.1038/s41565-021-00929-w.
Stabryla LM, Johnston KA, Diemler NA, Cooper VS, Millstone JE, Haig S-J, Gilbertson LM. Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions. Nature nanotechnology. 2021 Sep;16(9):996–1003.

Published In

Nature nanotechnology

DOI

EISSN

1748-3395

ISSN

1748-3387

Publication Date

September 2021

Volume

16

Issue

9

Start / End Page

996 / 1003

Related Subject Headings

  • Silver
  • Nanoscience & Nanotechnology
  • Microbial Sensitivity Tests
  • Metal Nanoparticles
  • Ions
  • Escherichia coli K12
  • Drug Resistance, Bacterial
  • Cell Movement