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c‐kit Gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors

Publication ,  Journal Article
KINOSHITA, K; ISOZAKI, K; HIROTA, S; NISHIDA, T; CHEN, HUI; NAKAHARA, M; NAGASAWA, Y; OHASHI, A; SHINOMURA, Y; KITAMURA, Y; MATSUZAWA, Y
Published in: Journal of Gastroenterology and Hepatology
February 2003

Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumors of the human gut. They frequently have gain‐of‐function mutations of the c‐kit gene, which encodes a receptor, tyrosine kinase. The mutations were found at exon 11 in most cases, and either at exon 9 or at exon 13 in rare cases. Recently, we found a family with multiple GIST and a gain‐of‐function mutation at exon 17. The family was the first reported GIST case with c‐kit gene mutation at exon 17 including sporadic GIST. Although we previously reported that the c‐kit gene mutation at exon 17 was not detected in 124 sporadic GIST by single‐strand conformation polymorphism (SSCP) analysis, the mutation at exon 17 observed in the familial GIST was detectable by the use of direct sequencing but not by our SSCP method. In the present study, we examined the mutations at exon 17 and exon 13 by using direct sequencing. Genomic DNA was extracted from formalin‐fixed, paraffin‐embedded GIST tissues. We could obtain 143 sporadic GIST cases appropriate for DNA analysis at exon 17 and 141 at exon 13. Exons 17 and 13 were amplified by using polymerase chain reaction and direct sequencing was conducted. No mutation was found at exon 17, and only one case with the mutation at exon 13 was observed. The GIST with the mutation at exon 13 was large and showed frequent mitosis, and the patient died of the recurrent GIST 3 years after the first operation. The mutation at exons 17 or 13 was considered to be very rare in sporadic GIST.

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Published In

Journal of Gastroenterology and Hepatology

DOI

EISSN

1440-1746

ISSN

0815-9319

Publication Date

February 2003

Volume

18

Issue

2

Start / End Page

147 / 151

Publisher

Wiley

Related Subject Headings

  • Gastroenterology & Hepatology
  • 1103 Clinical Sciences
 

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Chicago
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KINOSHITA, K., ISOZAKI, K., HIROTA, S., NISHIDA, T., CHEN, H. U. I., NAKAHARA, M., … MATSUZAWA, Y. (2003). c‐kit Gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors. Journal of Gastroenterology and Hepatology, 18(2), 147–151. https://doi.org/10.1046/j.1440-1746.2003.02911.x
KINOSHITA, K. A. Z. U. O., K. O. J. I. ISOZAKI, S. E. I. I. C. H. I. HIROTA, T. O. S. H. I. R. O. U. NISHIDA, H. U. I. CHEN, M. A. S. A. N. O. R. I. NAKAHARA, Y. U. T. A. K. A. NAGASAWA, et al. “c‐kit Gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors.” Journal of Gastroenterology and Hepatology 18, no. 2 (February 2003): 147–51. https://doi.org/10.1046/j.1440-1746.2003.02911.x.
KINOSHITA K, ISOZAKI K, HIROTA S, NISHIDA T, CHEN HUI, NAKAHARA M, et al. c‐kit Gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors. Journal of Gastroenterology and Hepatology. 2003 Feb;18(2):147–51.
KINOSHITA, K. A. Z. U. O., et al. “c‐kit Gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors.” Journal of Gastroenterology and Hepatology, vol. 18, no. 2, Wiley, Feb. 2003, pp. 147–51. Crossref, doi:10.1046/j.1440-1746.2003.02911.x.
KINOSHITA K, ISOZAKI K, HIROTA S, NISHIDA T, CHEN HUI, NAKAHARA M, NAGASAWA Y, OHASHI A, SHINOMURA Y, KITAMURA Y, MATSUZAWA Y. c‐kit Gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors. Journal of Gastroenterology and Hepatology. Wiley; 2003 Feb;18(2):147–151.
Journal cover image

Published In

Journal of Gastroenterology and Hepatology

DOI

EISSN

1440-1746

ISSN

0815-9319

Publication Date

February 2003

Volume

18

Issue

2

Start / End Page

147 / 151

Publisher

Wiley

Related Subject Headings

  • Gastroenterology & Hepatology
  • 1103 Clinical Sciences