A peptide sequence on carcinoembryonic antigen binds to a 80kD protein on Kupffer cells.
Publication
, Journal Article
Thomas, P; Petrick, AT; Toth, CA; Fox, ES; Elting, JJ; Steele, G
Published in: Biochem Biophys Res Commun
October 30, 1992
Clearance of carcinoembryonic antigen (CEA) from the circulation is by binding to Kupffer cells in the liver. We have shown that CEA binding to Kupffer cells occurs via a peptide sequence YPELPK representing amino acids 107-112 of the CEA sequence. This peptide sequence is located in the region between the N-terminal and the first immunoglobulin like loop domain. Using native CEA and peptides containing this sequence complexed with a heterobifunctional crosslinking agent and ligand blotting with biotinylated CEA and NCA we have shown binding to an 80kD protein on the Kupffer cell surface. This binding protein may be important in the development of hepatic metastases.
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Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
October 30, 1992
Volume
188
Issue
2
Start / End Page
671 / 677
Location
United States
Related Subject Headings
- Structure-Activity Relationship
- Rats, Sprague-Dawley
- Rats
- Peptides
- Molecular Weight
- Molecular Sequence Data
- Membrane Proteins
- Male
- Kupffer Cells
- Kinetics
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NLM
Thomas, P., Petrick, A. T., Toth, C. A., Fox, E. S., Elting, J. J., & Steele, G. (1992). A peptide sequence on carcinoembryonic antigen binds to a 80kD protein on Kupffer cells. Biochem Biophys Res Commun, 188(2), 671–677. https://doi.org/10.1016/0006-291x(92)91108-3
Thomas, P., A. T. Petrick, C. A. Toth, E. S. Fox, J. J. Elting, and G. Steele. “A peptide sequence on carcinoembryonic antigen binds to a 80kD protein on Kupffer cells.” Biochem Biophys Res Commun 188, no. 2 (October 30, 1992): 671–77. https://doi.org/10.1016/0006-291x(92)91108-3.
Thomas P, Petrick AT, Toth CA, Fox ES, Elting JJ, Steele G. A peptide sequence on carcinoembryonic antigen binds to a 80kD protein on Kupffer cells. Biochem Biophys Res Commun. 1992 Oct 30;188(2):671–7.
Thomas, P., et al. “A peptide sequence on carcinoembryonic antigen binds to a 80kD protein on Kupffer cells.” Biochem Biophys Res Commun, vol. 188, no. 2, Oct. 1992, pp. 671–77. Pubmed, doi:10.1016/0006-291x(92)91108-3.
Thomas P, Petrick AT, Toth CA, Fox ES, Elting JJ, Steele G. A peptide sequence on carcinoembryonic antigen binds to a 80kD protein on Kupffer cells. Biochem Biophys Res Commun. 1992 Oct 30;188(2):671–677.
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
October 30, 1992
Volume
188
Issue
2
Start / End Page
671 / 677
Location
United States
Related Subject Headings
- Structure-Activity Relationship
- Rats, Sprague-Dawley
- Rats
- Peptides
- Molecular Weight
- Molecular Sequence Data
- Membrane Proteins
- Male
- Kupffer Cells
- Kinetics