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Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring

Publication ,  Journal Article
Mondragon, P; Hwang, S; Schmid, A; Maupin-Furlow, JA
Published in: Bio-protocol
November 20, 2022

The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions. A common strategy used to investigate molecular mechanisms of stress response involves the deletion and/or site-directed mutagenesis of genes identified through omics studies followed by a comparison of the mutant and wild-type strains for phenotypic differences. The experimental methods used to monitor these differences must be controlled and reproducible. Current methods to examine recovery of halophilic archaea from extreme stress are complicated by extended incubation times, nutrients not typically encountered in the environment, and other related limitations. Here we describe a method for assessing the function of genes during hypochlorite stress in the halophilic archaeon Haloferax volcanii that overcomes these types of limitations. The method was found reproducible and informative in identifying genes needed for H. volcanii to recover from hypochlorite stress.

Duke Scholars

Published In

Bio-protocol

DOI

EISSN

2331-8325

Publication Date

November 20, 2022

Volume

12

Issue

22

Related Subject Headings

  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
 

Citation

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Mondragon, P., Hwang, S., Schmid, A., & Maupin-Furlow, J. A. (2022). Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring. Bio-Protocol, 12(22). https://doi.org/10.21769/BioProtoc.4557
Mondragon, P., S. Hwang, A. Schmid, and J. A. Maupin-Furlow. “Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring.” Bio-Protocol 12, no. 22 (November 20, 2022). https://doi.org/10.21769/BioProtoc.4557.
Mondragon, P., et al. “Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring.” Bio-Protocol, vol. 12, no. 22, Nov. 2022. Scopus, doi:10.21769/BioProtoc.4557.

Published In

Bio-protocol

DOI

EISSN

2331-8325

Publication Date

November 20, 2022

Volume

12

Issue

22

Related Subject Headings

  • 32 Biomedical and clinical sciences
  • 31 Biological sciences