Hyperpolarized ¹³C Magnetic Resonance Spectroscopic Imaging of Pyruvate Metabolism in Murine Breast Cancer Models of Different Metastatic Potential.

This study uses dynamic hyperpolarized [1-¹³C]pyruvate magnetic resonance spectroscopic imaging (MRSI) to estimate differences in glycolytic metabolism between highly metastatic (4T1, n = 7) and metastatically dormant (4T07, n = 7) murine breast cancer models. The apparent conversion rate of pyruvate-to-lactate (k_PL) and lactate-to-pyruvate area-under-the-curve ratio (AUC_L/P) were estimated from the metabolite images and compared with biochemical metabolic measures and immunohistochemistry (IHC). A non-significant trend of increasing k_PL (p = 0.17) and AUC_L/P (p = 0.11) from 4T07 to 4T1 tumors was observed. No significant differences in tumor IHC lactate dehydrogenase-A (LDHA), monocarboxylate transporter-1 (MCT1), cluster of differentiation 31 (CD31), and hypoxia inducible factor-α (HIF-1α), tumor lactate-dehydrogenase (LDH) activity, or blood lactate or glucose levels were found between the two tumor lines. However, AUC_L/P was significantly correlated with tumor LDH activity (ρ_spearman = 0.621, p = 0.027) and blood glucose levels (ρ_spearman = -0.474, p = 0.042). k_PL displayed a similar, non-significant trend for LDH activity (ρ_spearman = 0.480, p = 0.114) and blood glucose levels (ρ_spearman = -0.414, p = 0.088). Neither k_PL nor AUC_L/P were significantly correlated with blood lactate levels or tumor LDHA or MCT1. The significant positive correlation between AUC_L/P and tumor LDH activity indicates the potential of AUC_L/P as a biomarker of glycolytic metabolism in breast cancer models. However, the lack of a significant difference between in vivo tumor metabolism for the two models suggest similar pyruvate-to-lactate conversion despite differing metastatic potential.

Duke Scholars

Author Erin Macdonald Radiology